The invention provides a method for producing pleocidin by adopting
saccharopolyspora spinosa through
fermentation. According to the method,
saccharopolyspora spinosa Z68 is adopted as a
fermentation strain, an improved
fermentation medium is adopted, fermentation cultivation is carried out under the conditions that the temperature is 26-30 DEG C, the rotation speed is 150-220 rpm, the compression-ventilation ratio is 1 to (0.3-0.7), the pot pressure is 0.03-0.04 MPa, and the dissolved
oxygen is controlled to be 40% or above, and pleocidin is extracted from the fermentation liquid. According to the method provided by the invention,
staring from the physicochemical properties of the fermentation medium of
saccharopolyspora spinosa, the fermentation medium with stable yield of pleocidin and little foaming amount, and a material supplementation method are provided. The
soybean meal / soybean cake
powder in the fermentation medium are replaced by high-temperature soybean cake
powder, and the experiment proves that in the fermentation process, the high-temperature soybean cake
powder has small possibility of foaming compared with the
soybean meal and low-temperature soybean cake powder, while part of fat not available in the high-temperature soybean cake powder but available in the
soybean meal and the low-temperature soybean cake powder can be added from the outside in the middle and later periods of fermentation, and the adding at the time can achieve the effect of eliminating foams and solve the problem that the
carbon source is insufficient in the later period.