Method for producing pleocidin by adopting saccharopolyspora spinosa through fermentation

Abstract

The invention provides a method for producing pleocidin by adopting saccharopolyspora spinosa through fermentation. According to the method, saccharopolyspora spinosa Z68 is adopted as a fermentation strain, an improved fermentation medium is adopted, fermentation cultivation is carried out under the conditions that the temperature is 26-30 DEG C, the rotation speed is 150-220 rpm, the compression-ventilation ratio is 1 to (0.3-0.7), the pot pressure is 0.03-0.04 MPa, and the dissolved oxygen is controlled to be 40% or above, and pleocidin is extracted from the fermentation liquid. According to the method provided by the invention, staring from the physicochemical properties of the fermentation medium of saccharopolyspora spinosa, the fermentation medium with stable yield of pleocidin and little foaming amount, and a material supplementation method are provided. The soybean meal / soybean cake powder in the fermentation medium are replaced by high-temperature soybean cake powder, and the experiment proves that in the fermentation process, the high-temperature soybean cake powder has small possibility of foaming compared with the soybean meal and low-temperature soybean cake powder, while part of fat not available in the high-temperature soybean cake powder but available in the soybean meal and the low-temperature soybean cake powder can be added from the outside in the middle and later periods of fermentation, and the adding at the time can achieve the effect of eliminating foams and solve the problem that the carbon source is insufficient in the later period.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for producing spinosyn by fermentation of Saccharopolyspora spinosa. Background technique [0002] Spinosyn is a secondary metabolite produced by the aerobic fermentation of Saccharopolyspora spinosa, and belongs to macrolide compounds. The effective active components in its commercial products are spinosad A (85-90%) and spinosad Gericidin D (10-15%). Spinosad has no antibacterial activity, but it has good insecticidal activity. It has a wide insecticidal spectrum against pests of Lepidoptera and Thysanoptera. Lepidopteran pests have a good control effect. Spinosad is one of the most selective compounds among existing insecticides in the control of Lepidoptera pests, and its activity is equivalent to cypermethrin. While spinosyn has high insecticidal activity, it exhibits low toxicity to non-target organisms. It has low toxicity to mammals, birds and beneficial i...

AI Technical Summary

Problems solved by technology

Mechanical defoaming is generally inefficient and does not eliminate the root cause of foam

However, commercially available chemical defoamers (polyether defoamers, silicone defoamers, higher alcohol defoamers, etc.) have a greater impact on the fermentation of spinosad. In addition, the addition of a large amount of defoamers It also brings difficulties to the follow-up spinosad extraction work

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