2results about How to "Solve easy adhesion" patented technology

The application belongs to the technical field of biology, and discloses a method for rapidly dissociating zebrafish larvae intestine for single-cell sequencing, which comprises the following steps: anesthetizing zebrafish larvae, placing the zebrafish larvae in an inclined manner, and sucking off the anesthetic; and dissecting and separating from the abdominal swelling part to obtain the zebrafish larvae intestine. The method of the application omits the step of fixing the zebrafish larvae by using an insect needle, optimizes the removal mode of the anesthetic, adopts the inclined plate and the syringe suction mode, effectively reduces the movement of the zebrafish larvae, and does not need the positioning mode of fixing the zebrafish larvae by using the insect needle, thereby simplifying the operation process. The application significantly improves the processing efficiency and is better in repeatability and generalizability. Since the positioning, liquid suction and dissection modes adopted are more standardized, the application is more conducive to the reproduction and general application among laboratories.

The application discloses a rare earth up-conversion red-green-blue fluorescent coding microsphere, adopts a modified olefin-norbornene copolymer as a matrix material, and adopts red, blue or green rare earth up-conversion luminescent nanocrystals as a fluorescent coding material. The application further discloses a preparation method of the rare earth up-conversion red-green-blue fluorescent coding microsphere. The modified olefin-norbornene copolymer is blended with the up-conversion luminescent nanocrystals to obtain a uniform blend, and the blend is hot-pressed into a rod-shaped core layer which is inserted into a cladding material to form a preform rod, and then the preform rod is hot-drawn into a fiber, and then heat treatment is carried out to obtain a polymer fluorescent microsphere with a coding function. The method can control the fluorescent type and intensity of the fluorescent coding microsphere and the particle size of the microsphere according to requirements, the preparation process is simple, the method is suitable for large-scale production, the fluorescent performance is stable, and the method can be used for detecting cells, proteins or other molecules in biological samples.