The invention relates to a production method of organic bio-fertilizer. The method comprises the following steps: organic substances such as bean dregs, tobacco waste, scum, turf, animal protein, hoof meal, bone ash, pork skin, shell powder, and the like are prepared into powdery substances and evenly mixed, and then are fermented and decayed by using fermenting micro-organisms, after the content of the flora reaches 3 billion per gram, the inorganic elements such as N, P, K, and the like are added, the powdery substances are prepared into granular substances and dried, the dried granules together with bacteria strain, chitin and abamectin are mixed with water to form a package, and a product is formed after dehydration. The invention has the advantages that the method improves the quality of crops, reduces harmful substances and the content of heavy metal, improves the utilization ratio of the fertilizer, reduces the environmental pollution, improves the soil and cultivates the land capability.