The invention discloses a graphene heating ink, a preparation method and application thereof. The graphene heating ink comprises the following raw materials by mass percent: 5-15% of carbon black, 10-25% of graphite, 0.1-5% of graphene powder, 0.1-8% of carbon nanotube powder, 25-40% of an adhesive, 1-5% of an assistant, and the balance a mixed solvent. Through combination of dotted carbon black,linear carbon nanotubes and planar graphene, a mutually cross-linked stable structure can be formed inside the ink, and the safety is high. Through improvement of the preparation process and the ink formula components, blistering does not occur easily in the stirring process, and the ink has good adhesion during printing, the electro-thermal conversion efficiency of the heating ink is remarkably improved, the far infrared normal emissivity of an electrothermal film is significantly enhanced, the service life of the electrothermal film is greatly increased, and the power change of 100,000h aging test is less than 2.6%. Also, the implementation process adopted by the invention is simple, efficient and practical.