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Preparation method and application of semi-dry nitration microbial agent

A technology of nitrifying bacteria and nitrifying bacteria, applied in the field of preparation of semi-dry nitrifying bacteria, can solve the problems of nitrification system collapse, harsh living conditions, slow growth, etc., achieve low dosage, good stability, and improve ammonia nitrogen The effect of degradability

Active Publication Date: 2019-03-19
山东海景天环保科技股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrifying bacteria are strictly aerobic autotrophic bacteria with harsh living conditions and slow growth. They are often impacted in sewage treatment biochemical systems where water quality and quantity fluctuate frequently, causing the nitrification system to collapse.

Method used

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  • Preparation method and application of semi-dry nitration microbial agent
  • Preparation method and application of semi-dry nitration microbial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Preparation of a semi-dry nitrifying bacteria agent, take 20L of activated sludge in the aerobic tank of the sewage treatment plant under normal operation, let it stand for 2 hours, discard the supernatant, and then put the precipitated activated sludge under the speed of 2000r / min Centrifuge for 2 minutes, discard the supernatant to obtain concentrated activated sludge with a volume of 1L;

[0040] Prepare 9L special inorganic salt medium for nitrifying bacteria, the formula is: sodium bicarbonate 1.0g / L, dipotassium hydrogen phosphate trihydrate 1.0g / L, potassium dihydrogen phosphate 1.0g / L, sodium chloride 0.1g / L, seven Water ferrous sulfate 0.02g / L, anhydrous magnesium sulfate 0.02g / L, copper sulfate 0.02g / L, ammonium sulfate 0.5g / L, water to make up the balance.

[0041] The concentrated activated sludge is added to the medium to start the enrichment and cultivation of nitrifying bacteria. The enrichment culture method is as follows: use an electromagnetic aeratio...

Embodiment 2

[0047] Preparation of a semi-dry nitrifying bacteria agent, take 50L of activated sludge in the aerobic tank of the sewage treatment plant under normal operation, let it stand for 2 hours, discard the supernatant, and then put the precipitated activated sludge at a speed of 3000r / min Centrifuge for 3min, discard the supernatant, and obtain concentrated activated sludge with a volume of 3L;

[0048] Prepare 7L special inorganic salt medium for nitrifying bacteria, the formula is: sodium bicarbonate 1.2g / L, dipotassium hydrogen phosphate trihydrate 1.5g / L, potassium dihydrogen phosphate 1.3g / L, sodium chloride 0.4g / L, seven Water ferrous sulfate 0.02g / L, anhydrous magnesium sulfate 0.04g / L, copper sulfate 0.02g / L, ammonium sulfate 2.0g / L.

[0049] The concentrated activated sludge is added to the medium to start the enrichment and cultivation of nitrifying bacteria. The enrichment culture method is as follows: use an electromagnetic aeration pump to aerate the 10L medium to mai...

Embodiment 3

[0056] An application of a semi-dry nitrifying bacteria agent. The nitrifying bacteria agents prepared in Example 1 and Example 2 were stored for 12 months and taken out, and a certain tannery sewage was used as the treatment object to verify the nitrification effect of the bacteria agent. Establish 3 aerobic systems respectively, add 10L of tannery sewage respectively, use electromagnetic aeration pump for aeration, maintain dissolved oxygen at 4.0-6.0 mg / L; keep pH at 7.5-8.0.

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Abstract

The invention belongs to the technical fields of biology and water treatment and provides a preparation method and application of a semi-dry nitration microbial agent. The preparation method comprisesthe specific preparation steps: (1) adding aerobic tank active sludge of a sewage treatment plant in a normal working state as a bacteria source into a special inorganic salt culture medium for nitrifying bacteria, and carrying out enrichment culture; (2) after the enrichment culture is finished, carrying out habituated culture, so as to obtain a culture solution with high nitrifying activity; (3) concentrating the culture solution, adding the culture solution into protecting liquid with a certain concentration, and carrying out culturing for a proper period of time; (4) uniformly mixing themixed liquid obtained in the step (3) with processed carriers, so as to obtain the microbial agent; and (5) putting the microbial agent into a vacuum sealing bag, carrying out vacuum sealing, and putting the vacuum sealing bag in a shade place, and carrying out normal-temperature storage. When the microbial agent is used, a user only needs to disperse the microbial agent with water and adding themicrobial agent into an aerobic biochemical system; the microbial agent has the characteristics of high activity and tolerance, good stability, strong stress resistance and the like and further has the advantages of convenience in transportation and storage, small occupied space and the like; and the microbial agent does not need to be excited and rejuvenated in use.

Description

technical field [0001] The invention belongs to the technical field of biology and water treatment, and provides a preparation method and application of a semi-dry nitrifying bacteria agent. Background technique [0002] Ammonia nitrogen has become one of the ubiquitous pollutants in the water environment, which endangers the safety of aquatic organisms and even the entire ecosystem. There are physical, chemical and biological methods for denitrification. The biological method is widely used in sewage treatment because of its advantages of simple operation, low investment, low cost, no secondary pollution, and good treatment effect. Nitrifying bacteria are the microorganisms that play a major role in the biological denitrification process. The stability of nitrification and the improvement of nitrification rate are the key factors affecting the denitrification efficiency of the whole system. Nitrifying bacteria are strictly aerobic autotrophic bacteria with harsh living con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C12N1/02
CPCC12N1/02C12N1/20C12N1/36
Inventor 王珍马韵升栾波张映徐泽平吴文雷杨传伦车树刚薛飞
Owner 山东海景天环保科技股份公司
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