Unlock instant, AI-driven research and patent intelligence for your innovation.
A strain of Pediococcus pentosaceus and its application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Pediococcus pentosaceus and strains, applied in the field of microorganisms, can solve problems such as mammalian constipation, and achieve the effect of remarkable effect, high industrial application value, and intestinal laxative effect.
Active Publication Date: 2021-10-08
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
View PDF11 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] The purpose of the present invention is to provide a strain of Pediococcus pentosaceae and its application, and develop a bacterial strain with strong laxative effect and strong inhibitory ability to Staphylococcus aureus and Vibrio vulnificus to solve the problem of mammalian constipation , to provide more raw material options for the development of antibacterial drugs, health food or general food against Staphylococcus aureus and Vibrio vulnificus
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0021] Embodiment 1 Pediococcus pentosacea ( Pediococcus pentosaceus ) Isolation and identification of B49
[0022] 1. Strain isolation
[0023] Colostrum was obtained from healthy women in Fujian, China, who gave birth to a baby no more than 14 days old. The fresh colostrum was placed in a clean bench and diluted with sterile PBS solution. The undiluted milk and the diluted milk were distributed to MRS culture respectively. Base. Cultivate at a constant temperature at 37°C until colonies appear. Use an inoculation needle to pick out well-growing bacteria on the edge and inoculate them on a new MRS plate medium. After the newly inoculated bacteria grow into colonies, pick the endogenous bacteria on the edge. Bacteria were streak cultured and purified repeatedly until a single colony was obtained, which was named strain B49.
[0024] The formula of MRS liquid medium is: tryptone 10.0 g / L, beef powder 5.0 g / L, yeast powder 4.0 g / L, glucose 20.0 g / L, Tween 80 1.0 mL / L, K 2 H...
Embodiment 2
[0029] Embodiment 2 Pediococcus pentosacea ( Pediococcus pentosaceus ) Utilization of B49 to carbohydrates
[0030] Application of API50CHL kit (Biomerieux, France) to detect the utilization of sugars by the bacterial strain B49 isolated in Example 1, the results show that: Pediococcus pentosaceae B49 of the present invention can utilize L-arabinose, D-xylose, ribose, D - Glucose, D-mannose, D-fructose, D-galactose, D-lactose, D-cellobiose, maltose, trehalose, D-gentiobiose.
[0032] Application of APIZYM kit (Biomerieux, France) to detect the enzymatic activity of the isolated bacterial strain B49 in Example 1, the results show that: Pediococcus pentosaceae B49 of the present invention can secrete leucine aryl amidase, valine aryl amidase, Acid phosphatase, naphthol-As-diphosphate hydrolase, β-glucosidase, N-acetyl-β-glucosaminidase, cystine aryl amidase.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
diameter
aaaaa
aaaaa
Login to View More
Abstract
The invention discloses a strain of Pediococcus pentosaceae, the preserved name of the strain is Pediococcus pentosaceae B49, which was preserved in the General Microorganism Center of China Committee for the Preservation of Microorganisms on June 19, 2018, and the preservation number is: CGMCC No. 15957. Also disclosed is the application of the Pediococcus pentosaceae in the preparation of laxative medicine, health food or common food; the application in the preparation of anti-staphylococcus aureus medicine, health food or common food; , health food or general food applications. The Pediococcus pentosaceae B49 provided by the present invention has high safety. The strain and the fermented yoghurt prepared by using the strain can significantly relieve constipation. During in-depth research, it was surprisingly found that the strain has a very significant inhibitory effect on the growth of the pathogenic bacteria Staphylococcus aureus, and can significantly improve the survival rate of zebrafish after being challenged by Vibrio vulnificus.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Pediococcus pentosaceae and its application. Background technique [0002] With the change of eating habits and the continuous acceleration of the pace of life, constipation has become a common disease among middle-aged and elderly people and office workers, and it tends to become younger. According to statistics, the incidence of constipation in my country ranges from 3.4% to 27.2%. Long-term constipation can cause carcinogens produced by the fermentation of intestinal bacteria to induce intestinal cancer. Some patients with severe constipation have affected their quality of life. However, most patients with constipation are troubled by constipation, rarely go to the hospital, and pay less attention to it. Some patients take laxatives or traditional Chinese medicine enemas in order to solve the difficulty in defecation, but this will cause relatively large ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More