A working electrode for detecting hypoxanthine and xanthine and its enzyme biosensor

A working electrode, hypoxanthine technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, instruments, etc., can solve the problems of increasing cost, time, and complicated steps.

Active Publication Date: 2021-09-14
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Current conventional detection methods, such as high performance liquid chromatography, spectrophotometry, chemiluminescence, capillary electrophoresis, etc., can detect hypoxanthine and xanthine with high sensitivity and high selectivity; for example, Chinese invention patent application publication number CN106855508A discloses a A method for detecting hypoxanthine content in fish, the method is to take fish sample liquid, use horseradish peroxidase and xanthine oxidase to couple and catalyze the sample liquid, and then use ultraviolet spectrophotometer to detect and obtain hypoxanthine content content; however, the steps of these detection methods are very cumbersome, and require relatively expensive equipment and professional skilled technicians to operate, increasing the cost and time of detection

Method used

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  • A working electrode for detecting hypoxanthine and xanthine and its enzyme biosensor
  • A working electrode for detecting hypoxanthine and xanthine and its enzyme biosensor
  • A working electrode for detecting hypoxanthine and xanthine and its enzyme biosensor

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Experimental program
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Effect test

Embodiment 1

[0029] Preparation of working electrodes for enzymatic biosensors for the detection of hypoxanthine and xanthine

[0030] The preparation method comprises the following steps;

[0031] Step 1), preparing a composite of the copper metal organic framework;

[0032] First, providing a solution of xanthine oxidase and a copper metal-organic framework;

[0033] The xanthine oxidase used in this example was purchased from the product model XU-4500 of Sigma Company; 30 g of xanthine oxidase was added to 1 L of deionized water to prepare xanthine oxidase with a concentration of 30 mg / ml solution;

[0034] Regarding copper metal organic framework materials (hereinafter referred to as Cu-MOF), you can directly purchase commercially available products, or you can prepare them yourself, as long as the copper metal organic framework materials meet the following parameters: the specific surface area ranges from 30 to 80m 2 / g, the pore size of the surface pores is 1.7-50nm, and the surfa...

Embodiment 2

[0046] System of enzyme biosensors for detection of hypoxanthine and xanthine

[0047] A general enzyme biosensor system includes an electrochemical workstation and a reference electrode, a contrast electrode and a working electrode connected to the electrochemical workstation.

[0048] The electrochemical workstation acts as a monitor for signal changes in the entire reaction system. The electrochemical workstation used in this example was purchased from CHI 760 model of Shanghai Chenhua Company.

[0049] The working electrode of the enzyme biosensor system in Example 2 is the working electrode prepared in Example 1 above.

[0050] In this embodiment, the reference electrode can be a saturated calomel electrode (purchased from Ada Hengsheng Company), and the contrast electrode can be a platinum wire electrode (purchased from Gaoshi Ruilian Company).

[0051] In addition, regarding the reference electrode and the comparison electrode, those skilled in the art can also use ot...

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Abstract

The invention relates to a working electrode for detecting hypoxanthine and xanthine, a preparation method thereof, and an enzyme biosensor system using the working electrode; the preparation method of the working electrode of the present invention is simple, and the working electrode is compatible with participating The enzyme biosensor system composed of the ratio electrode, the comparison electrode and the electrochemical workstation can be used to detect hypoxanthine and xanthine, and has high accuracy. It can not only detect low-concentration substrates, but also detect hypoxanthine. It has high sensitivity to the concentration change of hypoxanthine and xanthine, and has a wide detection range. It can quickly and efficiently obtain the concentration detection results of hypoxanthine and xanthine, and has good stability. It can be used repeatedly. The sensitive detection of the substrate can be maintained even after repeated use.

Description

technical field [0001] The invention relates to a working electrode for detecting hypoxanthine and xanthine, a preparation method thereof, and an enzyme biosensor system using the working electrode, belonging to the technical field of food detection. Background technique [0002] China is a big ocean fishing country. Countless fish are caught and sold all over the world every year. After the fish are caught, they are prone to die. After the fish die, a series of complex chemical reactions will occur, including protein Decomposition, dissolution and lipolysis, etc., and even cause spoilage. [0003] After fish die, some catabolites will be produced, such as hypoxanthine and xanthine (the main catabolites of adenosine triphosphate (ATP) known), but long-term consumption of foods containing a large amount of hypoxanthine and xanthine will lead to hypoxanthine and xanthine. The accumulation of xanthine and xanthine in the human body leads to diseases such as gout, xanthuria, hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30C12Q1/00
CPCC12Q1/005G01N27/308G01N27/3277G01N27/3278
Inventor 张绫芷王治潘
Owner NINGBO UNIV
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