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A detection method of antibacterial and antifungal agents in bamboo and wood products

A detection method and technology of anti-fungal agent, applied in the field of detection, can solve the problems of inability to monitor bamboo and wood food related products, etc., and achieve the effect of improving the recovery rate

Active Publication Date: 2021-06-25
GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the current detection means can only detect several conventional antibacterial preservatives but cannot effectively monitor related bamboo and wood food-related products, the present invention provides a detection method for antibacterial and antifungal agents in bamboo and wood products method, which can simultaneously determine a variety of antibacterial preservatives in bamboo and wood food related products

Method used

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  • A detection method of antibacterial and antifungal agents in bamboo and wood products
  • A detection method of antibacterial and antifungal agents in bamboo and wood products
  • A detection method of antibacterial and antifungal agents in bamboo and wood products

Examples

Experimental program
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Effect test

Embodiment 1

[0044]Determination of antibacterial preservatives in bamboo chopsticks in Jiangmen City, Guangdong Province

[0045] Take an appropriate amount of solid sample, cut and grind the solid sample into particles with a particle size of less than 5mm*5mm, weigh 4.00g (accurate to 0.01g) of the crushed sample in a 50mL centrifuge tube, add 20mL of methanol, and tighten the cap. Vortex and mix, soak for 60 minutes, ultrasonically extract for 10 minutes, centrifuge at 3000r / min for 5 minutes, transfer the supernatant to a 50mL volumetric flask, repeat the extraction once, set the volume to the mark, transfer to a 125mL chicken heart bottle and evaporate with a rotary evaporator To near dryness, dilute to 1mL with methanol, pass through a 0.45μL organic filter membrane, enter into a high-performance liquid chromatograph, and measure at wavelengths of 230nm and 254nm, respectively.

[0046] Detection conditions include chromatographic column: Agilent ZORBAX SB-C18; mobile phase A is amm...

Embodiment 2

[0050] Determination of Antibacterial Preservatives in Bamboo Steamers in Luoding City, Guangdong Province

[0051] Take an appropriate amount of solid sample, cut and grind the solid sample into particles with a particle size of less than 5mm*5mm, weigh 4.00g (accurate to 0.01g) of the crushed sample in a 50mL centrifuge tube, add 20mL of methanol, and tighten the cap. Vortex and mix, soak for 60 minutes, ultrasonically extract for 10 minutes, centrifuge at 3000r / min for 5 minutes, transfer the supernatant to a 50mL volumetric flask, repeat the extraction once, set the volume to the mark, transfer to a 125mL chicken heart bottle and evaporate with a rotary evaporator To near dryness, dilute to 1mL with methanol, pass through a 0.45μL organic filter membrane, enter into a high-performance liquid chromatograph, and measure at wavelengths of 230nm and 254nm, respectively.

[0052] Detection conditions include chromatographic column: Agilent ZORBAX SB-C18; mobile phase A is ammon...

Embodiment 3

[0056] Determination of Antibacterial and Preservatives in Bamboo Cutting Boards in Luoding City, Guangdong Province

[0057] The detection method of this embodiment is roughly the same as the detection method in Example 2, except that the gradient elution conditions of the mobile phase are different, and the gradient elution conditions of the mobile phase are shown in Table 3. Table 3 is the ratio adjustment situation of mobile phase A and mobile phase B at each time point in embodiment 3.

[0058]

[0059] The test results see Figure 5 and Figure 6 . Figure 5 Be the high-performance liquid chromatogram of the present embodiment at a wavelength of 230nm; Figure 6 It is the HPLC chromatogram of the present embodiment at a wavelength of 254nm.

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Abstract

The invention relates to the technical field of detection, in particular to a method for detecting antibacterial and antifungal agents in bamboo and wood products. The invention comprises the following steps: cutting and shearing the solid sample into particles with a particle diameter less than 5mm*5mm, weighing 4g of the pulverized sample in a 50mL centrifuge tube, adding 20mL of methanol, tightening the lid, vortex mixing, soaking After 60 minutes, ultrasonically extract for 10 minutes, centrifuge at 3000r / min for 5 minutes, pipette the supernatant into a 50mL volumetric flask, repeat the extraction once, set the volume to the mark, transfer to a 125mL chicken heart bottle, evaporate to nearly dryness with a rotary evaporator, and dilute with methanol. The volume was reduced to 1 mL, passed through a 0.45 μL organic filter membrane, and input into a high-performance liquid chromatograph equipped with a diode array detector for detection. The detection method provided by the invention can quickly and accurately detect 15 kinds of antibacterial and antifungal agents in bamboo and wood products, thereby strengthening the effective monitoring of bamboo and wood food related products.

Description

technical field [0001] The invention belongs to the technical field of detection, in particular to a method for detecting antibacterial and antifungal agents in bamboo and wood products. Background technique [0002] The antibacterial and antifungal agent is actually a fungicide, and its mechanism of action mainly has three aspects: inhibiting protein synthesis, causing the bacteria to coagulate; inhibiting the synthesis of fungal ergocranol, inactivating enzymes; hindering metabolic functions, inhibiting spore production or spore production sprout. The scope of application of antifungal agents is expanding day by day, and the requirements for environmental safety in countries around the world are becoming increasingly stringent. As a good industrial antifungal agent, it must have the following items: high efficiency, broad spectrum, strong antibacterial power, and effective against a variety of mold rot fungi ; Low toxicity, safe and reliable to humans, animals and the sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李聪伍志航周铭林廖文斌程吉祥
Owner GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION
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