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A kind of orchid plant artificial seed embedding method
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An artificial seed and plant technology, applied in botany equipment and methods, seed coating/seed dressing, plant growth regulator, etc., can solve the problem of reducing the lifespan of somatic embryos, poor ventilation of alginate capsules, and affecting respiratory metabolism and other problems, to achieve the effect of saving medium cost, reducing seedling cost and simple production process
Active Publication Date: 2021-08-03
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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At present, the embedding method of artificial seeds is the most commonly used hydrogel sodium alginate coating technology. Alginate capsules have poor air permeability, inhibit the respiration of somatic embryos, and reduce the lifespan of somatic embryos (Sun Jingsan, edited by Gui Yaolin. Plant Cell Engineering Experimental Technology. Beijing: Science Press, 1990: 233.), very small and fragile orchid seeds are embedded in sodium alginate endosperm particles, which will obviously affect respiratory metabolism due to insufficient oxygen supply
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Embodiment 1
[0042] The fruits of Dendrobium officinale were washed with water, soaked in 75% alcohol in an ultra-clean workbench for instant sterilization, then soaked in 10% calcium hypochlorite solution for 20 minutes, and rinsed twice with sterile water.
[0043]Gelling agent sodium alginate concentration 4% (W / V), thickener sodium carboxymethyl cellulose 1.0% (W / V), nutritional labeling and hormone are MS+NAA0.5 mg / L+coconut juice 20% ( V / V)+sucrose 2.0% (W / V), mixed with conventional autoclaving, cooled to obtain a sol, cut the fruit of Dendrobium candidum on an ultra-clean workbench, shaken off the seeds to the sol, added 0.3 grams of seeds per liter of sol, Put it aside for sterile culture for 1 week, the seeds swell and turn yellow-green, add oxygen release agent ammonium persulfate in a sterile environment, the concentration is 0.2%, adjust the pH to 5.6, stir to suspend the seeds evenly in the endosperm, and immediately suck it up with a straw Sodium alginate sol mixed with seed...
Embodiment 2
[0046] The fruit of Bletilla striata (Bletilla striata) was washed with water, soaked in 75% alcohol in an ultra-clean workbench for instant sterilization, then soaked in 10% calcium hypochlorite solution for 20 minutes, and rinsed twice with sterile water.
[0047] The gelling agent sodium alginate concentration is 3%, the thickening agent polyvinylpyrrolidone is 1.5%, the nutrient composition is MS+sucrose 3.0% (W / V), mixing conventional autoclaving, cooling to obtain a sol, and cutting the bletilla striata in an ultra-clean workbench For the fruit, shake off the seeds into the sol, add 0.4 grams of seeds per liter of sol, let stand for aseptic culture for 15 days, the seeds swell and turn yellow-green, add oxygen-releasing agent hydrogen peroxide in a sterile environment, concentration 0.1%, percarbonate Sodium 0.1%, adjust the pH to 5.6, stir to suspend the seeds evenly in the embryo emulsion, immediately use a straw to absorb the sodium alginate sol containing the seeds, d...
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Abstract
The invention discloses a method for embedding artificial seeds of Orchidaceae plants, which comprises the following steps: 1) preparing an endosomal sol, and the said endosulcus sol comprises 1-10% of a gelling agent and a thickening agent according to the mass-volume ratio 0.5-5% and the nutrients and / or hormones required for seed germination; 2) Take the seeds out of the fruit, put them into the endosomal sol, and let them stand for sterile cultivation until the seeds absorb water and swell; 3) Add oxygen release agent 0.01 ‑1%, stir to suspend the seeds evenly in the endosperm, immediately use a straw to absorb the endosperm containing the seeds, drop it into the endosperm hardening solvent, and let it stand until the endosperm particles harden into artificial seeds. The invention has simple process and low cost.
Description
technical field [0001] The invention belongs to the technology of raising seedlings of plant seeds in the field of agriculture and forestry, and in particular relates to a method for embedding orchidaceae plant seeds to make artificial artificial seeds. Background technique [0002] Artificial seeds are the tissues and organs (somatic embryos, buds, callus, protocorms and other propagules) produced in in vitro plant culture that can develop into complete plants, and are embedded in artificial outer shells that contain nutrients and have protective functions. Granules formed within the membrane. Most orchids have high ornamental and medicinal values, and are popular plants for artificial seed production. The fruit of Orchidaceae contains thousands of seeds, the seeds are small or powdery, and the so-called embryo is actually dozens of undifferentiated cell clusters, and there is no endosperm tissue. Orchidaceae seed aseptic sowing (non-symbiotic germination) has many report...
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