Unlock instant, AI-driven research and patent intelligence for your innovation.
A kind of preparation method of chronic wound repair matrix
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A chronic wound and matrix technology, applied in the field of tissue engineering repair and medical biomaterials, can solve the problems of low immunity, long healing period, and perennial non-healing.
Active Publication Date: 2021-09-10
山东隽秀生物科技股份有限公司
View PDF9 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Chronic wounds are affected by tissue hypoxia, ischemia, infection, reduction in the number of local growth factors, and low autoimmunity. The healing cycle is long, and even perennial non-healing has brought a great impact on the lives of patients.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0019] Example 1: A preparation method of chronic wound repair matrix
[0020] Take 5-month-old fetal cowhide, cut into small pieces no larger than 2mm, wash 3 times with purified water, freeze the washed fetal cowhide in liquid nitrogen at -196°C for 24 hours, take it out, thaw it in a constant temperature water bath at 37°C, and wash it with purified water. Wash 3 times, drain and transfer to 5% TritonX-100—PBS solution, soak for 1 hour, and then perform ultrasonic treatment, the ultrasonic power is 90KW, and the working time is 5 minutes. After the ultrasonic treatment is completed, wash 3 times with purified water. Repeat the above steps 1 time.
[0021] Soak the decellularized fetal cowhide matrix obtained after the above treatment in 15%~18% peracetic acid solution, use high-speed shearing equipment to homogenate, stir until it forms a gel-state solution, and use electrospinning equipment to prepare Microspheres, the coagulation bath is 2 times the PBS solution, the tem...
Embodiment 2
[0022] Example 2: A preparation method of chronic wound repair matrix
[0023] Take 3-month-old fetal cowhide, cut it into small pieces no larger than 2mm, wash 3 times with purified water, put the washed fetal cowhide in the freezer of -20°C refrigerator for 24 hours, take it out, thaw it in a constant temperature water bath at 37°C, and purify Wash with water for 3 times, drain and transfer to 3% Triton X-100-PBS solution, soak for 1 hour, and then perform ultrasonic treatment. The ultrasonic power is 90KW, and the working time is 5 minutes. Repeat the above steps 1 time.
[0024] Soak the decellularized fetal cowhide matrix obtained after the above treatment in 15%~18% peracetic acid solution, use high-speed shearing equipment to homogenate, stir until it forms a gel-state solution, and use electrospinning equipment to prepare Microspheres, the coagulation bath is 5 times PBS solution, the temperature of the coagulation bath is 37 ° C, wherein, the coagulation bath also co...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a preparation method of a matrix for chronic wound repair, comprising the following steps: (1) taking 3-5 month-old fetal cowhide, cutting it into pieces, and removing the cell structure therein through freezing-thawing, ultrasonic wave combined with chemical elution, etc. (2) Using electrospinning technology, the decellularized fetal bovine skin extracellular matrix and montmorillonite powder are made into microspheres, and dried at low temperature to make a chronic wound repair matrix. The chronic wound repairing matrix prepared by the invention can effectively absorb deep wounds and keep the wounds clean; at the same time, the active biological factors contained in the fetal bovine skin extracellular matrix are helpful for tissue regeneration and repair.
Description
technical field [0001] The invention belongs to the fields of medical biomaterials and tissue engineering repair, and in particular relates to a chronic wound repair matrix and a preparation method thereof. Background technique [0002] With the aging of the population and the increase of diabetes and obesity worldwide, the incidence of chronic wounds is also increasing. The common chronic wounds in clinical practice include trauma infection, pressure sores, diabetic ulcers, venous ulcers and so on. Chronic wounds are affected by tissue hypoxia, ischemia, infection, reduction in the number of local growth factors, and low autoimmunity. The healing cycle is long, and even perennial non-healing has brought a great impact on the lives of patients. The emergence of tissue engineered matrix provides a new solution for chronic wound treatment. [0003] Tissue engineering is a new interdisciplinary science based on cell biology and material science. Its core elements are cells, sc...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More