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Detection marker of steroid-induced osteonecrosis of femoral head necrosis and application thereof

A technology for femoral head necrosis and hormones, applied in the direction of microbial determination/inspection, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of collecting specimen marker kits, etc.

Inactive Publication Date: 2019-04-02
陈雷雷
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Problems solved by technology

However, most of the current research is only on the expression level of exons rather than the gene expression of all transcriptomics, and most of the studies have not collected clinical samples for verification and marker kits again. Therefore, for the research on the markers of SIONFH, we need to further screen

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  • Detection marker of steroid-induced osteonecrosis of femoral head necrosis and application thereof
  • Detection marker of steroid-induced osteonecrosis of femoral head necrosis and application thereof
  • Detection marker of steroid-induced osteonecrosis of femoral head necrosis and application thereof

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Embodiment 1

[0037] 1 Experimental method:

[0038] 1.1 Collection of blood PBMC from SIONFH patients and healthy volunteers

[0039] RNA-seq sequencing stage: screening 4 cases of steroid-induced femoral head necrosis in SLE (systemic lupus erythematosus) who visited the Department of Orthopedics and Joints of a hospital in Guangzhou from September 2015 to May 2016 and met the inclusion criteria. There were 4 cases of osteonecrosis of the femoral head, including 4 healthy volunteers, 7 males and 13 females, aged 20 to 50 years, with an average age of 35 years. The general information of the 12 clinical samples used in the RNA-seq sequencing stage is shown in Table 1.

[0040] Verification stage: Screening 12 cases of SLE with steroid-induced necrosis of the femoral head and SLE without steroid-induced femoral head necrosis who visited the Department of Orthopedics and Joints of a hospital in Guangzhou from December 2015 to December 2016 and recruited healthy volunteers There were 12 case...

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Abstract

The present invention discloses a detection marker of steroid-induced osteonecrosis of femoral head necrosis and an application thereof. RNA expression levels of 6 genes of NPNT, KDM5D, BOLA2, EIFYAY,RPS4Y1 and PPKY are down-regulated in SIONFH patients for the first time. The detection marker lays a good foundation on preparing a kit for diagnosis or auxiliary diagnosis of SIONFH.

Description

technical field [0001] The invention specifically relates to a detection marker of steroid-induced necrosis of the femoral head and its application. Background technique [0002] In clinical work, femoral head necrosis has become a common problem faced by orthopedic surgeons around the world. Difficulties mainly focus on two aspects: early diagnosis and specific pathogenesis. Since no sensitive markers for patients with SIONFH have been found and the specific mechanism of femoral head necrosis progression has not yet been clarified, so if we want to make new breakthroughs in the diagnosis and treatment of SIONFH disease, we must diagnose and treat necrosis at an early stage. The specific pathogenic mechanism will continue to be studied in depth. [0003] It is imagined that if the latest RNA-seq technology can be used to sequence the peripheral blood PBMCs of patients with steroid-induced necrosis of the femoral head in the early stage, find the differential genes, and aft...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158
Inventor 陈雷雷孙友强何伟洪郭驹张庆文刘予豪王海彬
Owner 陈雷雷
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