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Rapid thermal cycling for sample analyses and processing

A thermal cycle and thermal curve technology, which is applied in the preparation of test samples, analytical materials, biomass post-processing, etc., can solve the troublesome and expensive problems of installing reactors

Inactive Publication Date: 2019-04-02
STAR ARRAY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of long tubular reactors not only makes the installation and operation of a large number of reactors cumbersome, but also expensive
Achieving heating uniformity across all reactors can be challenging when reactors are in large arrays or panels

Method used

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  • Rapid thermal cycling for sample analyses and processing
  • Rapid thermal cycling for sample analyses and processing
  • Rapid thermal cycling for sample analyses and processing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 11

[0101] Embodiment 1.1: nucleic acid analysis method, comprising the following steps: adding at least one reaction material 21 into at least one reactor 15, sealing the reactor 15, amplifying nucleic acid, using heating and / or underheating during nucleic acid amplification A method that describes at least one heating bath at a temperature above or below a target temperature required for nucleic acid amplification. When the nucleic acid is being amplified, the reactor 15 is arranged to alternately enter at least two heating baths with different temperatures for thermal cycling. When the temperature of the reactor 15 is close to or equal to the target temperature required for nucleic acid amplification, the reactor 15 is quickly moved from the heating bath where the reactor 15 is located to another heating bath. When superheating and / or underheating methods are used for nucleic acid amplification in three steps, the temperature of heating bath I is set higher than the target temp...

Embodiment 12

[0102] Example 1.2: The difference between this example and Example 1.1 is that when the temperature of the reactor 15 reaches the target temperature required for nucleic acid amplification, the reactor 15 is removed from the heating bath I and quickly transferred to the heating bath II .

Embodiment 13

[0103] Embodiment 1.3: The difference between this embodiment and Embodiment 1.1 is that when the temperature of the reactor 15 exceeds the target temperature required for nucleic acid amplification, the reactor 15 quickly moves from the heating bath III to the heating bath IV, and the heating bath IV The temperature is set to the desired target temperature for the extension.

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Abstract

Apparatus and method for thermal processing of nucleic acid in a thermal profile is provided. The apparatus employs a reactor holder for holding reactor(s) each accommodating reaction material containing the nucleic acid. The apparatus comprises a first bath; and a second bath, bath mediums in the baths being respectively maintainable at two different temperatures THIGH and TLOW; and a transfer means for allowing the reactor(s) to be in the two baths in a plurality of thermal cycles to alternately attain: a predetermined high target temperature THT, and a predetermined low target temperature TLT, while the apparatus adapts to a temperature-offset feature defined by at least one condition from the group consisting: a) the THT is lower than the THIGH, b) the TLT is higher than the TLOW, andc) the conditions at a) and b).

Description

technical field [0001] The present invention relates to methods and devices for carrying out nucleic acid amplification reactions in samples. Background technique [0002] Polymerase chain reaction (PCR) is increasingly important for molecular biology, food safety, and environmental monitoring. Many biological researchers use PCR in their nucleic acid analysis work due to its high sensitivity and specificity. The time period of PCR is usually about 1 hour, mainly due to the time-consuming PCR thermocycling process, which is suitable for heating and cooling the reactor containing the sample to different temperatures for DNA denaturation, annealing and extension. Typically, thermal cycling devices and methods employ moving the reactor between two heating baths set at the desired target temperature for the nucleic acid amplification reaction. Researchers have been working to increase the speed of thermal cycling. [0003] Thermoelectric coolers (TEC) or Peltier coolers are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L7/02B01L7/00B01L9/06C12M1/38C12M1/34C12Q1/686
CPCG01N1/44G01N21/6402G01N21/6486B01L7/02B01L9/06B01L2300/0618B01L2300/0858B01L2400/065G01N2035/00386B01L2300/0816B01L2300/0829B01L2300/0832B01L7/5255G01N2035/00366B01L9/065B01L2200/08B01L2200/141B01L2200/147B01L2300/12B01L2300/1805B01L2300/185C09K5/14B01F31/20B01L3/5082C12Q1/686G01N35/04B01L3/5027B01L2200/06B01L2300/0627B01L2300/1894B01L2200/14B01L7/52B01L2200/025B01L2300/1827C12Q1/6848G01N35/00584G01N2035/00396G01N2035/0412
Inventor 龚海庆文燕曾旭东
Owner STAR ARRAY