Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection and authentication method for ribavirin

A ribavirin and aqueous solution technology, applied in the field of substance detection and identification, can solve the problems of high raw material requirements, high production technology and cost requirements, and achieve the effect of obvious phenomenon, sensitive response and strong specificity

Active Publication Date: 2019-04-05
GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the requirements for raw materials are high, and the production technology and cost are relatively high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection and authentication method for ribavirin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Capsules (tested by LC-MS, this product contains ribavirin chemical composition)

[0048] Take 1 capsule of the sample to be tested, take about 1 capsule content, put it into a 10ml test tube with a stopper, add 2ml of pure water, shake for 3 minutes, let it stand for 2 minutes, take a 3ml needle-free syringe, pull out the piston, and filter with a 0.45mm mesh Pour about 2ml of the upper liquid of the stoppered test tube into a syringe, squeeze it with a piston, and collect the filtrate into a 5ml centrifuge tube. Add 1 ml of dichloromethane to the above 5 ml centrifuge tube. Cover the centrifuge tube cap and turn it upside down 10 times (do not shake vigorously). After standing for 2min, the layers were separated. Add dropwise 0.5 ml of ferrous sulfate solution with a mass percent concentration of 10% to the upper layer solution of the above-mentioned 5 ml centrifuge tube. After shaking a few times, the liquid in the upper layer of the solution was yellow.

[0049]...

Embodiment 2

[0050] Example 2: Capsules (tested by LC-MS, this product does not contain ribavirin chemical components)

[0051] Take 3 samples of the sample to be tested, put about 3 contents into a 10mL stoppered test tube, add 4mL of pure water, cover the lid, shake for 3min, let it stand for 2min, take a 3ml needle-free syringe, pull out the plunger, connect 0.45 mm mesh filter, pour about 2ml of the upper liquid of the stoppered test tube into a syringe, squeeze it with a piston, and collect the filtrate into a 5ml centrifuge tube. Add 1 ml of dichloromethane to the above 5 ml centrifuge tube. Cover the centrifuge tube cap and turn it upside down 10 times (do not shake vigorously). After standing for 2min, the layers were separated. Add dropwise 0.5ml of ferrous sulfate solution with a concentration of 15% by mass to the upper layer solution of the above 5ml centrifuge tube. After a few shakes, the upper layer of the solution did not appear light yellow or yellow.

[0052] Experime...

example 3

[0053] Example 3: Tablet (tested by LC-MS, this product contains ribavirin chemical components)

[0054] Take 1 piece of sample, grind it finely, put it in a 10mL test tube with a stopper, add 4mL of pure water, cover it, shake it for 1min, let it stand for 1min, take a 3ml needle-free syringe, pull out the piston, and filter with a 0.45mm mesh Pour about 2ml of the upper liquid of the stoppered test tube into a syringe, squeeze it with a piston, and collect the filtrate into a 5ml centrifuge tube. Add 1 ml of dichloromethane to the above 5 ml centrifuge tube. Cover the centrifuge tube cap and turn it upside down 10 times (do not shake vigorously). After standing for 2min, the layers were separated. Add dropwise 0.5ml of ferrous sulfate solution with a concentration of 15% by mass to the upper layer solution of the above 5ml centrifuge tube. After shaking a few times, the liquid in the upper layer of the solution was light yellow.

[0055] Experimental results: The upper l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a detection and authentication method for ribavirin. The method comprises the following steps that: a sample to be detected is dissolved in water to obtain the aqueous solutionof the sample to be detected; dichloromethane is added into the aqueous solution to carry out layering after shaking and standing; ferrous sulfate solution is added into the layered aqueous solution;and the color of supernatant is observed, and when the supernatant is yellow, the sample contains the ribavirin. The method has the advantages of sensitive reaction, high specificity and obvious result phenomenon, does not need to be equipped with an instrument, and is suitable for field quick detection.

Description

technical field [0001] The invention relates to the technical field of substance detection and identification, in particular to a ribavirin detection and identification method. Background technique [0002] Ribavirin is a synthetic nucleoside antiviral drug, which is a common broad-spectrum antiviral drug with a wide range of effects and definite curative effect. [0003] The publicity of "pure natural, good effect and no adverse reaction" in commercially available health food makes consumers quite favor it. However, in order to improve the so-called antiviral efficacy, some unscrupulous companies illegally add ribavirin to health care products to increase efficacy and seek economic benefits. In this way, the public will take a large amount without knowing it, which will seriously endanger public health and safety. [0004] For the illegal addition of ribavirin in Chinese patent medicines and health foods, there are mainly two detection methods at this stage. [0005] 1. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 钟汉高吕丽珊杨鹏博杨松林张祺
Owner GUANGZHOU ANNUO FOOD SCI & TECH CO LTD