Progesterone immunochromatographic test strip and manufacturing method thereof
A technology of immunochromatographic detection and production method, which is applied in the field of progesterone immunochromatographic detection test strips and its production, and can solve problems such as low sensitivity, narrow linear range, and insufficient accuracy
Active Publication Date: 2019-04-09
海卫特(广州)医疗科技有限公司
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Problems solved by technology
In the immunochromatographic detection of small molecule substances, there are common problems of low sensitivity, narrow linear range and insufficient accuracy
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preparation example Construction
[0055] The above-mentioned preparation process of the labeling pad and the detection membrane can be performed in any order. Similarly, the preparation steps of the coupling complex of the progesterone monoclonal antibody and the first complex and the activated fluorescent latex particle solution are also not limited in order, and can be specifically produced according to needs. If it can be prepared at the same time.
[0056] In a specific example, the concentration of activated fluorescent latex particles in the solution of activated fluorescent latex particles is 800 μg / 100 μl to 1200 μg / 100 μl, such as 800 μg / 100 μl, 900 μg / 100 μl, 1000 μg / 100 μl, 1100 μg / 100 μl or 1200 μg / 100 μl, etc. .
[0057] When adding the coupling complex to the activated fluorescent latex particle solution, it is added to the activated fluorescent latex particle solution in an amount of 5 μg to 20 μg per 100 μl of the activated fluorescent latex particle solution, such as per 100 μl of the activate...
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Abstract
The invention relates to a progesterone immunochromatographic test strip and a manufacturing method thereof. A progesterone monoclonal antibody and a first coordinate compound are compounded to form acoupled complex, and the coupled complex is connected with a fluorescent latex particle to form a fluorescent latex particle marked coupled complex. When the fluorescent latex particle marked coupledcomplex is combined with a progesterone antigen in a sample, a progesterone antigen-fluorescent coupled complex can be formed; through a chromatographic function, the progesterone antigen-fluorescentcoupled complex is specifically bond with a coated second coordinate compound in a quality control area of a detection film for massive accumulation; the other non-bound fluorescent latex particle marked coupled complex which is not bond with the progesterone antigen is bond with a coated progesterone coupled antigen in a detection area of the detection film; and the mode that the detection areaand the quality control area share one fluorescent latex particle is capable of amplifying the semaphore of a reaction system and effectively solving the problem that the binding between the monoclonal antibody and the fluorescent latex particle is not uniform, thereby greatly improving the sensitivity and correctness and broadening the linear range.
Description
technical field [0001] The invention relates to the field of immunochromatographic detection, in particular to a progesterone immunochromatographic detection test strip and a manufacturing method thereof. Background technique [0002] Progesterone (English Progesterone, referred to as Prog, also known as progesterone, progesterone, progesterone, luteinizing hormone, progesterone, progesterone or progesterone in Chinese) is a drug that involves the female menstrual cycle, pregnancy and Steroids that affect human or animal embryos are the most important progestins. The progesterone test is mainly used to understand the function of the corpus luteum and whether the ovaries are ovulating. In non-pregnant females, progesterone is secreted in large quantities by the corpus luteum of the ovary only in the second half of each menstrual cycle. It is also secreted by the brain, liver and adrenal glands. During pregnancy (starting from the third month), the placenta can be secreted ...
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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 何浩王伟许荣黄灿岑赞询
Owner 海卫特(广州)医疗科技有限公司