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Nucleic acid reagent, kit, system and method for detecting human papillomavirus

A papilloma and human detection technology, applied in the biological field, can solve the problems of high cost and cross contamination

Active Publication Date: 2019-04-12
北京卓诚惠生生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread application at home and abroad in recent years, some clinical shortcomings and problems have gradually been exposed, such as easy cross-contamination, high cost, cross-reaction between high-risk and low-risk types, and foreign There are individual high-risk missed detection rates reported in the literature

Method used

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  • Nucleic acid reagent, kit, system and method for detecting human papillomavirus
  • Nucleic acid reagent, kit, system and method for detecting human papillomavirus
  • Nucleic acid reagent, kit, system and method for detecting human papillomavirus

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Experimental program
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Embodiment

[0074] 1. Synthesis of primers and probes

[0075] According to the primer sequence shown in Table 1 and the probe sequence shown in Table 2, sequence synthesis was performed. In the sequence, Y represents the degenerate base T / C; R represents the degenerate base A / G; W represents the degenerate base A / T; in the probe, FAM is 6-carboxyfluorescein, JOE is 2,7-two Methyl-4,5-dichloro-6-carboxyfluorescein, TAMRA is 6-carboxytetramethylrhodamine, and CY5 is 5H-indole cyanine. The brackets in the probe sequence of Table 2 indicate that the t on the left side of the brackets has a fluorescent label, and the content in the brackets indicates the choice of fluorescent label.

[0076] Table 1

[0077]

[0078]

[0079] Table 2

[0080]

[0081]

[0082] 2. Sample processing

[0083] After the patient’s cervical swab samples are collected by conventional methods, the exfoliated cervical cells on the swabs are collected using the sampler matched with ParaDNA, and directly placed in the ParaDNA ...

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Abstract

The present disclosure relates to a nucleic acid reagent, kit, system and method for detecting human papillomavirus. The nucleic acid reagent comprises primers shown as SEQ ID NO.1-50 and probes shownas SEQ ID NO.53-77, wherein the primers and probes are stored separately from each other or arbitrarily mixed with each other. According to the primers and probes described above, the nucleic acid reagent, kit, system and method for detecting the human papillomavirus are established, and can realize rapid, comprehensive, sensitive, specific and automatic detection result determination.

Description

Technical field [0001] The present disclosure relates to the field of biotechnology, in particular to a nucleic acid reagent, kit, system and method for detecting human papillomavirus. Background technique [0002] With the popularity of screening, the incidence and mortality of cervical cancer in developed countries or regions have dropped significantly. Some developed countries such as the United Kingdom and Australia have reduced the incidence of cervical cancer by 33% from 1991 to 2000. Because screening in underdeveloped areas has not been carried out or is not standardized worldwide, cervical cancer is the second most common malignant tumor in women, and the mortality rate remains high. The cause of cervical cancer is clear. A large number of studies have shown that persistent high-risk human papillomavirus (HPV) infection is the main cause of cervical cancer and precancerous lesions. [0003] HPV belongs to the family of Papovavirdae (Papovavirdae) papillomavirus. HPV mainl...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6858C12N15/11C12M1/38C12M1/34C12R1/93
CPCC12Q1/6858C12Q1/708C12Q2600/156C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2527/107C12Q2563/107
Inventor 王雷马寅佳林笑冬王晓艳张志强
Owner 北京卓诚惠生生物科技股份有限公司