Fluorescent immunochromatographic test strip capable of rapid GFAP detection and preparation and application of test strip

Abstract

The invention relates to a fluorescent immunochromatographic test strip capable of rapid GFAP detection and preparation and application of the test strip. The test strip comprises a base plate, and asample pad, a bonder release pad, a chromatographic film and a water absorption pad arranged along the length direction of the base plate respectively; the chromatographic film is provided with a detection line and a quality control line separate from the detection line; a fluorescent probe of a GFAP monoclonal antibody marked by fluorescent microballoons is fixed on the bonder release pad; the detection line is coated with a GFAP antigen paired with the microballoon mark antibody; and the quality control line is coated with an IgG antibody. Compared with the prior art, the fluorescent immunochromatographic test strip capable of rapid GFAP detection is prepared by preparing the GFAP recombined antigen and monoclonal antibody and combining a time resolved fluorescence lateral immunochromatography technique; and a method for rapidly detecting a cerebral injury marker GFAP in serum is formed, so that the concentration of GFAP in the human serum can be detected rapidly and quantitatively.

Description

technical field [0001] The invention belongs to the technical field of fluorescent immunoassay, and in particular relates to a fluorescent immunochromatographic test strip for rapidly detecting GFAP and its preparation and application. Background technique [0002] Traumatic brain injury (traumatic brain injury, TBI) is the most common disease in neurosurgery, and it is one of the main causes of death and disability worldwide; its incidence and fatality rate ranks third, second only to cancer and cardiovascular disease; leading cause of traumatic death. According to the order of occurrence, TBI can be divided into primary brain injury and secondary brain injury. Primary brain injury refers to the injury that occurs immediately when violence directly or indirectly acts on the head, and the pathological changes include contusion, laceration, and bleeding. Secondary brain injury refers to brain injury lesions that occur after injury, involving changes in cells and molecules, ...

AI Technical Summary

Problems solved by technology

[0006] In 2017, the U.S. FDA approved Banyan Biomarker’s GFAP as a diagnostic reagent for TBI. However, its detection method is in the ELISA mode. This technology is effective in principle, but the operation is complicated and time-consuming

[0007] Patent CN 107102146A discloses a method for the quantitative detection of GFAP in serum based on carbon dots as fluorescent markers

Images