Chute plate apparatus and assay method for studying the effect of fluid shear stress on cells

Abstract

A chute plate device and measurement method for studying the influence of fluid shear stress on cells, comprising a box shell (1) for accommodating cell carriers, arranged on the box shell (1) and used for adjusting the box shell (1) ) into the input tube (5) for injecting the flow fluid that produces shear stress on the cells, and the output tube that is arranged on the box shell (1) and is used to discharge the flow fluid that produces shear stress on the cells in the box shell (1) (6), the signal pick-up sensor (7), which is arranged in the output tube (6) and is used to pick up the signal of the shear stress acting on the cell, passes through the box shell (1) to accommodate the cell carrier, and passes through the input tube ( 5) and the output tube (6), to realize the flow fluid that produces shear stress on the cells, and the signal pickup sensor (7), to pick up the signal used to pick up the shear stress acting on the cells, no longer using a The flat plate is grooved, thus improving the precision of the effects of fluid shear stress on the cells.

Description

technical field [0001] The invention relates to a chute plate device and a measurement method, in particular to a chute plate device and a measurement method for studying the influence of fluid shear stress on cells. Background technique [0002] Studying the influence of fluid shear stress on cell morphology and function is an important research topic for cells. In the existing chute plate devices and measurement methods used to study the influence of fluid shear stress on cells, conventional chute plates are used. That is, a single flat plate tank, the steps are: the cells are seeded on the glass slide first, put into the flat plate groove, the liquid flows in from one end of the chute plate, passes through the plate, and flows out from the other end, according to the shape of the stressed cells and Functional changes to determine the effects of fluid shear stress on cells. Calculate the magnitude of the fluid shear stress generated according to the velocity and amount of...

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Problems solved by technology

[0004] 1. It is limited to a single type of cell and cannot be applied to the interaction of two different cells. Under the condition of fluid shear stress, the interaction between cells (cross talk) is a normal state in the body. Since there is no Corresponding instruments and equipment are used to study the interaction between cells. At present, the interaction between cells is only in a static state, and it is impossible to realize the interaction between two different cells under the condition of fluid shear stress.

[0005] 2. For the conventional chute plate used, the fluid shear stress cannot be precisely controlled. The fluid shear stress generated is only calculated according to the velocity and amount of the outflow liquid, which is not conducive to force control or experimental results. repeat

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