A kind of ointment with increasing white blood cell content and preparation method thereof

A technology of white blood cell content, applied in medical preparations containing active ingredients, extracellular fluid diseases, pharmaceutical formulations, etc., can solve the problems of many operation steps, large ethanol, consumption, etc. The effect of a wide source of raw materials

Active Publication Date: 2022-04-08
HUNAN BUTIAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of the above two processes require secondary alkalization and ethanol reaction system, and there are many operation steps and consume a large amount of ethanol

Method used

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  • A kind of ointment with increasing white blood cell content and preparation method thereof
  • A kind of ointment with increasing white blood cell content and preparation method thereof
  • A kind of ointment with increasing white blood cell content and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Described carboxymethyl tuckahoe polysaccharide is prepared by the following method:

[0032] S1. Cultivation of Poria cocos: Aseptically inoculate Poria cocos mycelium in wort liquid medium, and culture at 28°C for 4 days;

[0033] S2. Preparation of Poria cocos polysaccharide: The cultured Poria cocos medium was fermented at 35°C for 2 days, heated to a slight boil, extracted for 1 hour, filtered, and a mixed solvent of 3 times the amount of chloroform and n-butanol was added. The mixing volume ratio is 4:1, shake, centrifuge, add 3 times the amount of 95% ethanol, overnight, filter to get the precipitate, wash twice with ether to obtain Poria cocos polysaccharide;

[0034] S3. Preparation of Poria cocos polysaccharide: add 10 g of Poria polysaccharide to 100 mL of 0.5 mol / L sodium periodate solution for selective oxidation, adjust the pH to 3 with trifluoroacetic acid, moderately acidify, and the acid hydrolysis time is 30 min, filter , the solid was washed with wat...

Embodiment 2

[0038] Described carboxymethyl tuckahoe polysaccharide is prepared by the following method:

[0039] S1. Cultivation of Poria cocos: Aseptically inoculate Poria cocos mycelium in wort liquid medium, and culture at 28°C for 5 days;

[0040] S2. Preparation of Poria cocos polysaccharide: The cultured Poria cocos medium was fermented at 35°C for 2 days, heated to a slight boil, extracted for 2 hours, filtered, and a mixed solvent of 5 times the volume of chloroform and n-butanol was added. The mixing volume ratio is 6:1, shake, centrifuge, add 3 times the amount of 95% ethanol, overnight, filter to obtain the precipitate, wash with ether 3 times to obtain Poria cocos polysaccharide;

[0041] S3. Preparation of Poria cocos polysaccharide: add 10 g of Poria polysaccharide to 30 mL of 1.5 mol / L sodium periodate solution for selective oxidation, adjust the pH to 4 with trifluoroacetic acid, moderately hydrolyze with acid for 1 h, filter , the solid was washed with water to obtain th...

Embodiment 3

[0045] Described carboxymethyl tuckahoe polysaccharide is prepared by the following method:

[0046] S1. Cultivation of Poria cocos: Aseptically inoculate Poria cocos mycelium in wort liquid medium, and culture at 28°C for 4 days;

[0047] S2. Preparation of Poria cocos polysaccharide: the cultured Poria cocos medium was fermented at 35°C for 2 days, heated to a slight boil, extracted for 2 hours, filtered, and a mixed solvent of chloroform and n-butanol was added, and the mixed volume ratio of chloroform and n-butanol was At 5:1, shake, centrifuge, add 3 times the amount of 95% ethanol, overnight, filter to obtain a precipitate, wash with ether 3 times to obtain Poria cocos polysaccharide;

[0048] S3. Preparation of Poria cocos polysaccharide: add 10 g of Poria polysaccharide to 50 mL of 1.0 mol / L sodium periodate solution for selective oxidation, adjust the pH to 3.6 with trifluoroacetic acid, moderately acidolyse, the acidolysis time is 45min, filter , the solid was washe...

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Abstract

The present invention proposes a kind of ointment that can improve leukocyte content, and is prepared from the following raw materials by weight: 10-20 parts of carboxymethyl polysaccharide, 12-17 parts of colostrum basic protein, and 5-parts of wheat oligopeptide 10 parts, 35‑40 parts of mulberry, 5‑10 parts of longan meat, 10‑20 parts of coix seed, 20‑25 parts of sealwort, 10‑20 parts of yam, 20‑25 parts of jujube and 15‑20 parts of honey. The ointment of the invention has the effects of enhancing the body's immunity, increasing the leukocyte content in the blood, improving the body's ability to withstand hypoxia, and the like. It has definite curative effect on the treatment of diseases such as low immune function and leukopenia. The invention has wide sources of raw materials, simple preparation method, and the prepared ointment has a good effect of improving immunity, can increase the content of white blood cells, stimulate the proliferation of lymphocytes, reduce the incidence of body diseases, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine health care, in particular to a cream with the ability to increase the content of white blood cells. Background technique [0002] In recent years, the decline of the world's environmental quality and the increase of people's living pressure have led to an increasing number of people in sub-health state. At the same time, with the continuous improvement of living standards, people are paying more and more attention to personal health issues. With the enhancement of people's health awareness, people gradually began to choose healthy food and beverages. The market share of carbonated beverages has been declining due to this demand. Tea beverages, whole grain beverages, fruit juice beverages and functional beverages have begun to be favored by people. To enhance immunity, it is necessary to ensure the amino acid content of physical strength. The existence of amino acids in the hu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/01C08B37/00A61P7/00A61P37/04A61P39/00A61K31/715A61K35/644
Inventor 戴鑫汶戴甲木孙梦锦龙孝金邹奇张豪唐心文
Owner HUNAN BUTIAN PHARMA
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