Detection kit for aspartate aminotransferase with good repeatability

An aspartate amino acid detection kit technology, applied in the field of biochemical detection, can solve the problems of poor repeatability, unsatisfactory repeatability, and low samples, and achieve the effects of increasing permeability, improving repeatability, and improving uniformity

Inactive Publication Date: 2019-05-07
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] This detection method has the advantages of low cost, fast detection speed in large batches, and good accuracy. It has been widely used in clinical practice. In poor instruments and equipment, the problem of poor repeatability of performance will be more prominent. The samples of normal people are generally concentrated in the range of 12-40U / L, while in clinical practice, patient samples that are lower than the normal reference range are important for the detection of advanced liver tumors. Patient diagnosis has important reference significance, and the samples within the normal reference range are relatively low, so improving and improving the repeatability of the method products is also of great practical value
[0010] In response to this problem, the reasons for the unsatisfactory repeatability were explored in detail, and a good repeatable aspartate aminotransferase (AST) detection kit and method of use were invented.

Method used

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  • Detection kit for aspartate aminotransferase with good repeatability
  • Detection kit for aspartate aminotransferase with good repeatability
  • Detection kit for aspartate aminotransferase with good repeatability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) A common detection reagent for aspartate aminotransferase (AST) provided in this example, including reagent R1 and reagent R2, the specific composition:

[0031] Reagent R1:

[0032]

[0033] Reagent R2:

[0034]

[0035] 2) The usage method of the reagent of this embodiment:

[0036] A common aspartate aminotransferase (AST) detection kit described in this example uses a fully automatic biochemical analyzer with dual reagent functions, such as the Hitachi 7180 fully automatic analyzer, etc., using Rate A method to measure. Place R1 and R2 on the corresponding reagent positions according to the ratio of 4:1, and place distilled water, standards and samples on the corresponding positions of the sample plate. The operation is shown in Table 1.

[0037] Table 1 Reagent detection method table

[0038]

[0039] Calculation: aspartate aminotransferase (AST) content with good repeatability (U / L) = (ΔA determination ÷ ΔA standard) × C standard

Embodiment 2

[0041] A reproducible aspartate aminotransferase detection kit described in this example includes reagent R1 and reagent R2:

[0042] 1) The components of reagent R1 include:

[0043]

[0044]

[0045] Described reagent R2 comprises the component of following content:

[0046]

[0047] 2) The usage method of the reagent of this embodiment:

[0048] The reagents in this example are used with a fully automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 fully automatic analyzer, etc., and are measured by the Rate A method. Place R1 and R2 on the corresponding reagent positions according to the ratio of 4:1, and place distilled water, standards and samples on the corresponding positions of the sample plate. The operation is shown in Table 2.

[0049] Table 2 Reagent detection method table

[0050]

[0051] Calculation: aspartate aminotransferase (AST) content with good repeatability (U / L) = (ΔA determination ÷ ΔA standard) × C standard

Embodiment 3

[0053] A reproducible aspartate aminotransferase detection kit described in this example includes reagent R1 and reagent R2:

[0054] 1) The components of reagent R1 include:

[0055]

[0056] Described reagent R2 comprises the component of following content:

[0057]

[0058]

[0059] 2) The usage method of this embodiment is the same as that of embodiment 2.

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Abstract

The invention relates to the technical field of biochemical detection, in particular to a detection kit for aspartate aminotransferase with good repeatability and a preparation and application methodthereof. The kit comprises a reagent R1 and a reagent R2. The reagent R1 comprises a buffer solution, a PH value adjusting agent, L-aspartic acid, potassium ferrocyanide, an AP5A, an EGTA, lithium chloride, a surfactant, levoglucosides, lactate dehydrogenase, malate dehydrogenase, and a preservative component. The reagent R2 comprises a buffer solution, a PH value adjusting agent, a BSA, a preservative, a-ketoglutarate, and NADH components. The kit is optimized in terms of removing reflection interference, increasing the activity of the reacted enzyme, improving the uniformity of the reactionsystem, and preferably selecting the excellent sub-wavelength. The kit remarkably improves the repeatability of reagents, especially the repeatability of low-value samples so as to improve the accuracy of clinical diagnosis results and achieve great clinical practical value.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and in particular relates to a highly repeatable aspartate aminotransferase detection kit and its preparation and use methods. Background technique [0002] Aspartate aminotransferase (aspartate aminotransferase, AST) exists in various tissues of the human body, with the most abundant content in the myocardium, followed by the liver. Under normal circumstances, the content of AST in serum is very low. When the relevant tissues and organs are damaged, the concentration of AST in serum will change accordingly. [0003] Liver blood tests are the most common blood tests that can be used to evaluate liver function or liver damage. The most common laboratory test abnormality in patients with chronic hepatitis is elevated serum transaminase, commonly used serum aspartate aminotransferase (AST). AST is mainly distributed in liver cells, and a small part exists in muscle cells. If the liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 甘宜梧罗维晓王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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