Rayleigh scattering deduction method in three-dimensional fluorescence
A Rayleigh scattering and three-dimensional fluorescence technology, applied in the field of eliminating fluorescence Rayleigh scattering, can solve the problems of long measurement time and information loss, and achieve the effect of saving measurement time, reducing information loss, and reducing the risk of information loss.
Active Publication Date: 2019-05-14
GUANGXI UNIVERSITY OF TECHNOLOGY
View PDF5 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0003] The technical problem to be solved by the present invention is to provide a method for subtracting Rayleigh scattering in three-dimensional fluorescence, so as to solve the shortcomings of the prior art, such as long measurement time and high risk of information loss
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0063] The present invention selects the Rayleigh scattering deduction method in the three-dimensional fluorescence of rhodamine B as embodiment one, and this method comprises the following steps:
[0064] S1. Measure the three-dimensional fluorescence spectrum of the sample
[0065] figure 1 It is the EEM three-dimensional map of the wavelength range of 200-800nm, figure 2 is the local map of the main fluorescence peak;
[0066] image 3 Among them are the emission spectra corresponding to the excitation wavelengths of 636.6nm, 617.7nm and 598.8nm; most of the Rayleigh peaks do not overlap with the fluorescence and can be directly erased; only the part of the excitation spectrum where the Rayleigh peak overlaps with the fluorescence deal with.
[0067] For the serious overlapping of Rayleigh peak and fluorescence peak, such as Figure 4 , there are difficulties in both direct subtraction and interpolation methods. Next, taking the fluorescence emission spectrum excited ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a Rayleigh scattering deduction method in three-dimensional fluorescence, and relates to a method for eliminating fluorescent Rayleigh scattering. The method comprises the following steps: firstly, finding out a Rayleigh peak which is not overlapped with fluorescence to acquire Rayleigh peak data R; eliminating random errors and noises of the Rayleigh peak data R and usingthe Rayleigh peak data R as a unit Rayleigh peak RD; eliminating the Rayleigh peak gradually at the beginning of emission spectrum obtained by overlapping the Rayleigh peak with the fluorescence untilthe all overlapped Rayleigh scattering can be eliminated. According to the Rayleigh scattering deduction method in the three-dimensional fluorescence disclosed by the invention, the Rayleigh scattering of the three-dimensional fluorescence can be eliminated, so that the repeatability can be ensured, and repeated measurement blanks are also not needed, and thus, the measurement time is reduced, the loss risk of information quantity is also reduced, and the method is simple and convenient and is easy for being popularized.
Description
technical field [0001] The invention relates to a method for eliminating Rayleigh scattering of fluorescence, in particular to a method for subtracting Rayleigh scattering in three-dimensional fluorescence. Background technique [0002] Three-dimensional fluorescence spectroscopy has been widely used in recent years as a fingerprint and a qualitative and quantitative method. In order to improve the quality of the spectrum and make the qualitative and quantitative analysis more accurate, it is necessary to eliminate the Rayleigh scattering signal contained in the spectrum. Commonly used methods include: blank subtraction method, Delaunay triangle interpolation method, zeroing method and missing data reconstruction method (MDR), etc. There are two main ideas. One is the blank subtraction method, which eliminates the Rayleigh data and retains the fluorescence data from the mixed data containing Rayleigh and fluorescence. However, the 3D fluorescence measurement takes a long ti...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 姚志湘姚桔粟晖刘柳
Owner GUANGXI UNIVERSITY OF TECHNOLOGY