Flexible up-conversion blue light sensor capable of regulating cryptochrome CRY2 protein allosterism in vivo and preparation method thereof

Abstract

The invention discloses a flexible up-conversion blue light sensor capable of regulating morphochrome CRY2 protein allosterism in vivo and a preparation method thereof. The preparation method comprises the following steps of 1) preparation and characterization of a blue light up-conversion micro-rod doped with a rare earth thulium element; 2) preparation of the flexible up-conversion blue light sensor: (1) preparing a polydimethylsiloxane precursor of different ratios of 10:1-7:1, mixing the blue light up-conversion micro-rod doped with rare earth thulium element by 10-100mg/ml, vacuumizing for 30-40min at 0.03MPa-0.05MPa to remove bubbles and evenly spin coating the mixture on a polyester film; (2) after the liquid forms the film for 0.2mm-3mm, conducting vacuum curing at high temperatureof 80 DEG C for 30-60 min; (3) compacting the film after cooling to avoid generation of bubbles. The sensor has an area of 25mm<2>-1cm<2>, and can emit bright blue light with the wavelength of 470nmunder the excitation of 980nm near-infrared light, and the blue light emitted can change the structure phase of the cryptochrome CRY2 protein to achieve safe efficient light conversion of particles invivo.

Description

technical field [0001] The invention belongs to the field of biotechnology and material technology, and specifically relates to a flexible up-conversion blue light sensor capable of regulating the allosteric structure of cryptochrome CRY2 protein in vivo and a preparation method thereof. Background technique [0002] Up-conversion luminescent material is a material that absorbs long-wavelength light and emits short-wavelength light. Because of its long excitation light wavelength (980nm) and good tissue penetration, it is widely used in the treatment of living diseases , signaling pathway regulation, etc. The application methods of up-conversion materials in vivo mainly include local injection and tail vein injection, but both methods have limitations. First of all, the up-conversion material is composed of rare earth metals, and the safety of its application in living bodies needs to be carefully considered. Usually, complex surface modification methods are used to reduce...

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Problems solved by technology

Usually, complex surface modification methods are used to reduce its biological toxicity, such as citric acid surface modification, polyethylene glycol coating surface, etc., and excessive modification of up-conversion materials will affect its photon yield and greatly reduce the weaken its luminous efficiency; secondly, the up-conversion is gradually metabolized in the body through long-term blood circulation or body fluid circulation, and the commonly used method is multiple injections. large metabolic burden

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