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Application of 3-hydroxybutyric acid and derivatives thereof in preparation of anti-atherosclerosis products

A technology of atherosclerosis and hydroxybutyric acid, which is applied in the field of medicine, can solve the problems of great differences in the efficacy of arterial plaques and large side effects, and achieve the effect of good druggability, small side effects, and lowering blood lipids

Pending Publication Date: 2019-05-28
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have clear blood lipid-lowering effects, but their efficacy in inhibiting arterial plaques varies greatly, and long-term use has relatively large side effects

Method used

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  • Application of 3-hydroxybutyric acid and derivatives thereof in preparation of anti-atherosclerosis products
  • Application of 3-hydroxybutyric acid and derivatives thereof in preparation of anti-atherosclerosis products
  • Application of 3-hydroxybutyric acid and derivatives thereof in preparation of anti-atherosclerosis products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. 3-Hydroxybutyric Acid Reduces Cardiac Outflow Tract Plaque Area in Apoe Knockout Mice

[0037] The mouse aortic root was fixed in 4% paraformaldehyde, and the tissue was taken out to make paraffin sections for H&E staining. The specific steps are as follows: 1), select a paraffin section with a good shape under the microscope, first place it in a 65°C oven for 1-2 hours, 2), put it in an automatic dehydrator, dewax to water, 3), Cole's hematoxylin Stain for 2-3 minutes, 4), wash with water to remove the floating color, observe the staining of the cell nucleus under the microscope, if it is still light, you can extend the staining time with Cole's hematoxylin solution, 5), differentiate with 1% hydrochloric acid alcohol for 3-10 seconds, and observe under the microscope Observe the coloring of the cell nucleus. If the decolorization is too heavy, return to Cole's hematoxylin staining solution for restaining. 6) Rinse gently with running water for about 3 minut...

Embodiment 2

[0041] Example 2. 3-Hydroxybutyric acid reduces aortic plaque burden in apoe knockout mice

[0042] The whole mouse aorta (32 in total) was taken from the APOE knockout mice in the model group and the administration group, and fixed in 4% paraformaldehyde. After the aorta was washed twice with PBS, the general oil red O staining experiment was carried out. The steps are as follows: 1), take out the aortic tissue from the paraformaldehyde fixative solution, rinse with PBS solution to remove formaldehyde; 2), place the aorta under a stereo microscope, use micro scissors and micro tweezers, the two cooperate with each other , carefully remove the fat around the aorta and other tissues; 3), cut the aorta longitudinally along the aortic arch; Put the opened aorta into the oil red O working solution, and dip it at room temperature for 2-4 hours; 5), use 70% ethanol to decolorize 3-5 times, each time for 15 seconds, until there is no lesion and the blood vessel wall becomes transpare...

Embodiment 3

[0046] Example 3, 3-hydroxybutyric acid reduces blood lipids in apoe knockout mice

[0047] Before the mice were sacrificed, the blood was taken, and the serum was separated by centrifugation. The serum was tested with an automatic biochemical analyzer for four items of blood lipids: total cholesterol (TC), total triglycerides (TG), low-density lipoprotein cholesterol (HDL-C), high Density lipoprotein cholesterol (LDL-C).

[0048] Figure 5 Indicating that 3-hydroxybutyric acid can reduce blood lipids in apoe knockout mice. Among them, TC: total cholesterol, TG: total triglyceride, LDL-C: low-density lipoprotein cholesterol, HDL-C: high-density lipoprotein cholesterol, 3-HB 100mg / kg group can reduce TC, TG, LDL-C The content of p<0.05 has a significant difference. But there is not much change in HDL-C in serum.

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Abstract

The invention belongs to the field of medicine, and particularly relates to application of 3-hydroxybutyric acid and derivatives thereof in preparation of anti-atherosclerosis products. Compared withthe prior art, the application has the advantages that (1) the 3-hydroxybutyric acid and derivatives thereof can be used for preparing anti-atherosclerosis drugs or health products, blood lipid can bereduced, inflammatory reactions during the onset of atherosclerosis can be inhibited, an anti-atherosclerosis effect is achieved, and accordingly, the 3-hydroxybutyric acid and derivatives thereof have clinical application prospects; (2) the 3-hydroxybutyric acid and derivatives thereof have few side effects, and are particularly high in safety, suitable for long-term use and high in druggability.

Description

technical field [0001] The invention belongs to the field of medicine, and specifically relates to the application of 3-hydroxybutyric acid and its derivatives in the preparation of anti-atherosclerosis products. Background technique [0002] Preventing the occurrence of atherosclerosis (AS) and promoting the regression of arterial plaques are fundamental measures to reduce cardiovascular and cerebrovascular morbidity and mortality. Atherosclerosis is a common and most important type of arteriosclerotic vascular disease, which is characterized by the onset of arterial intima lesions, followed by lipid and complex carbohydrate accumulation / hemorrhage, thrombosis, and fibrous tissue hyperplasia and calcinosis, and there is gradual degeneration and calcification of the arterial media. The lesions often involve elastic and large and medium muscular arteries. The accumulation of lipids in the inner lining of the arteries takes on the appearance of a yellow atheroma, hence the na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/19A61K31/22A61P9/10A61P3/06A61P29/00
Inventor 陈国强张书杰李子华张雨点
Owner TSINGHUA UNIV
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