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A method for mass spectrometry imaging of forchlorfenuron in melon tissue

An imaging method and mass spectrometry imaging technology, applied in the field of mass spectrometry imaging, can solve the problems of target object interference, low target object response, inability to achieve precise positioning, etc., and achieve the effects of good retention, high slice integrity, and simple slice preparation method.

Active Publication Date: 2020-11-17
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the auxiliary matrix is ​​easy to interfere with the target, resulting in a low response of the target and unable to achieve precise positioning

Method used

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  • A method for mass spectrometry imaging of forchlorfenuron in melon tissue
  • A method for mass spectrometry imaging of forchlorfenuron in melon tissue
  • A method for mass spectrometry imaging of forchlorfenuron in melon tissue

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Raw material treatment: Melon samples were used in the greenhouse of the experimental base of the Chinese Academy of Agricultural Sciences from July to October 2018. They were divided into melons in the spraying group and melons in the control group. Dip it in the pyruronium solution for 3-5 seconds, and pick it after 2 hours; the melons in the blank control group are artificially pollinated on the day after the flowering of the young melon fruit, and pick it after 2 hours; after the melon fruit is picked, wrap it in tin foil and place it in liquid nitrogen Store in a -80°C freezer until use.

[0055] The mass spectrometry imaging method of forchlorfenuron in melon tissue provided by the invention comprises the following steps:

[0056] (1) After freezing the spare melon fruit, use a cryostat to slice it. The temperature of the cryostat is set at -18° C., and the thickness of the slice is 35 μm.

[0057] (2) Add 100 mg / mL forchlorfenuron standard solution dropwise on t...

Embodiment 2

[0078] According to the embodiment of the mass spectrometry imaging method for forchlorfenuron in melon tissue in Example 1, the difference is that in step (4), the matrix CHCA is selected, and the matrix is ​​coated by evaporation. In order to improve the response of forchlorfenuron, The substrate can also be coated using a two-step method, that is, the substrate CHCA is evaporated and then sprayed with 1 mL of methanol solution.

Embodiment 3

[0083] In the method of mass spectrometry imaging of forchlorfenuron in melon tissue:

[0084] Step (1) Select the melon slices of the blank control group and the treatment group respectively, and attach them to the same glass slide respectively.

[0085] In step (2), no standard substance was added dropwise on the melon in the control group.

[0086] In step (3), only the optical images of the whole muskmelon tissue sections of the control group and the treatment group were obtained, as shown in Figure 5 .

[0087] In step (4), CHCA was used as the substrate; the substrate coating method was a two-step method. First, CHCA was deposited using an evaporation device (iMLayer), and after evaporation, 1 mL of methanol solution was sprayed on melon tissue slices with a hand-held spray gun.

[0088] Step (5) Set the same primary and secondary mass spectrometry conditions in the control group and the application group, and perform laser irradiation.

[0089] Step (6) Extract the se...

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Abstract

The invention provides a mass spectrometry imaging method for forchlorfenuron in cucumis melon tissue, and belongs to the technical field of mass spectrometry imaging. The imaging method comprises thefollowing steps that the cucumis melon tissue is frozen, sliced, attached to conductive glass slides and naturally air-dried; optical images of air-dried cucumis melon tissue slices are collected; the air-dried cucumis melon tissue slices are evaporated with CHCA and sprayed with chromatographic-grade pure methanol; the cucumis melon tissue slices coated with a substrate are subjected to laser desorption ionization; through processing of data processing software, a corresponding mass spectrometry image of the target substance forchlorfenuron on the cucumis melon tissue slices is obtained andsuperposed with the optical image for comparison and analysis, and the spatial distribution condition of the forchlorfenuron in the cucumis melon tissue is obtained. The method is quick and convenientto implement without complex pre-processing techniques, the optical image and mass spectrometry information are integrated, the high-resolution morphological images are observed, molecular identification and visualized distribution analysis on the forchlorfenuron can be carried out, and a new method is provided for studying the spatial distribution of the forchlorfenuron in cucumis melon fruits.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry imaging, and in particular relates to a mass spectrometry imaging method for forchlorfenuron in melon tissue. Background technique [0002] Melon (Cucumis melon L.) is an important horticultural crop in the world. Because of its sweet fruit, strong aroma, rich in protein, carbohydrates, vitamins and other nutrients, it occupies an important position in the production and consumption of fruits and vegetables in my country. Forchlorfenuron is a phenylurea plant growth regulator with cytokinin activity. Due to the complex nature of melon itself and the limitation of cultivation environment conditions, forchlorfenuron is widely used as a fruit-setting agent in the cultivation of muskmelon in facilities. It has brought huge economic benefits to the majority of fruit farmers and enterprises, and the use of forchlorfenuron has also brought severe challenges and threats to the safety of agricult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N1/28
Inventor 金芬王琦邵华李春梅王静苏杭
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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