Preparative thin layer chromatograph and using method thereof

Abstract

The invention discloses a preparative thin layer chromatograph. The preparative thin layer chromatograph comprises a thin layer chromatograph plate (1), a developing cylinder (2) and a supporting rack(3), a plastic clamp (5) is clamped at the upper end of the thin layer chromatograph plate, two separated electrodes (6) are arranged at a clamping opening of the plastic clamp (5), and the plastic clamp (5) is connected with a stepping motor rotating shaft (8) through a flexible wire (7); the electrodes (6) and a stepping motor are each electrically connected with a circuit board (4). The thin-layer chromatograph plate (1) is a rectangular plate, one side face of the thin layer chromatograph plate (1) is coated with a stationary phase layer, the length of the stationary phase layer is 10-30cm, the width of the stationary phase layer is 10-30 cm, the thickness is 0.5-2.0 mm, and the two separated electrodes (6) at the clamping opening of the plastic clamp are tightly attached to the upper end of the stationary phase layer; the thin layer chromatograph plate (1) is perpendicularly arranged in the developing cylinder (2). The invention further discloses a using method of the preparative thin layer chromatograph.

Description

Technical field [0001] The invention belongs to the field of chemical instruments, and specifically relates to a preparative thin-layer chromatograph and a use method thereof. Background technique [0002] Preparative thin layer chromatography (TLC) is an important method for compound separation in organic chemistry. It has the advantages of simple operation and solvent saving. It is to coat a suitable stationary phase (such as silica gel) on a glass plate, plastic or aluminum substrate to form a uniform stationary phase layer to form a thin-layer chromatography plate; uniformly coat the sample to be separated on one end of the stationary phase to form a uniform strip ; Then use a suitable polar developing agent (the developing agent is usually a mixture of two or more organic solvents or pure water in different proportions, the organic solvents usually include methanol, acetone, ethyl acetate, etc.) to expand the sample strip ; Due to the different adsorption effects of the dev...

AI Technical Summary

Problems solved by technology

[0003] Although TLC is easy to operate, the rate of developer development cannot be determined

Scientific researchers often spend a lot of time waiting for the developer to expand to the set position; if the development of the developer cannot be closely monitored, it is often because the TLC plate is not removed from the developer in time, resulting in excessive development of the developer. , all samples are concentrated on the top of the TLC plate, which eventually leads to analysis failure; at the same time, even if the development of the developing agent is closely monitored, it is difficult to control the development to the same height each time, thus affecting the separation effect of the subsequent compounds, as well as the accuracy and accuracy of the analysis. accuracy

Images