A high-throughput two-phase aqueous droplet chip assisted by a pneumatic valve

Abstract

The invention provides a high-throughput two-phase aqueous droplet chip assisted by a pneumatic valve. The chip has a structure of upper and lower layers. , consisting of continuous phase inlet, continuous phase split channel and continuous phase split outlet; the lower layer is a functional chip, consisting of gas inlet gas split channel, gas channel, pneumatic pump valve, dispersed phase inlet, dispersed phase channel, continuous phase inlet, continuous phase channel , consisting of the main channel and the droplet outlet. The chip is based on the traditional "cross" microfluidic droplet chip and is made with an integrated pneumatic pump valve system. The invention can effectively promote and control the high-efficiency generation of two-phase water droplets. By adjusting the parameters such as the two-phase flow rate and the switch cycle of the pump valve, stable and uniform two-phase water droplets can be obtained. The chip is expected to play a role in biological applications such as protein separation, cell compartmentalized culture, and DNA extraction.

Description

technical field [0001] The invention belongs to the fields of microfluidic technology, material chemistry, biochemistry and the like, and in particular relates to a high-flux two-phase aqueous droplet chip assisted by a pneumatic valve. Background technique [0002] Microfluidic technology has the advantages of less sample consumption, high portability, high integration, and high detection sensitivity. It has been widely used in chemistry, biology, pharmacy, physics and other fields. Microfluidic droplet manipulation technology is one of the important branches, which can directly use the incompatibility between fluids to obtain monodisperse droplets, and manipulate them by capturing, sorting, splitting, and merging them. The obtained droplets have the advantages of small volume and large specific surface area, which can effectively promote material exchange and chemical reactions. Therefore, this type of chip has been widely used in small molecule detection, single cell ana...

AI Technical Summary

Problems solved by technology

However, there are also obvious disadvantages in the two-phase system: the surface energy between the two water phases is much smaller than that of the oil-water system, which makes the use of traditional droplet chips to produce two-phase droplets, especially the controllable generation of two-phase droplets. become a huge challenge; this also limits the application of microfluidic chips in this field

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