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SSR primer pair for identifying hybrid cotton variety gossypium arboreum 1279 and product and identification method thereof

A hybrid cotton and primer pair technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as the limitation of hybrid cotton, the interference of breeder judgment, and the large impact of field environment , to achieve strong specificity, stable and reliable detection

Active Publication Date: 2019-06-14
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, breeders often use traditional techniques and methods such as visual observation and long-term breeding experience to judge hybrid cotton. Larger, the annual field environment (disease, insect damage, rainfall, etc.) differences lead to large differences in cotton phenotypes, causing major interference to the breeder's judgment; Cotton is very limited

Method used

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  • SSR primer pair for identifying hybrid cotton variety gossypium arboreum 1279 and product and identification method thereof
  • SSR primer pair for identifying hybrid cotton variety gossypium arboreum 1279 and product and identification method thereof
  • SSR primer pair for identifying hybrid cotton variety gossypium arboreum 1279 and product and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Cotton material

[0067] Seeds of the following cotton varieties: Zhongmian 1279, Upland cotton genetic standard line TM-1, Zhongmian Institute 10, Lumianyan 28, Shumian 2, Chuanmian 239, Emian 18 and Simian 2, Zhongmian Institute 12, Zhongmian Institute 49, Xinlu Zhong 69.

[0068] 2. DNA extraction

[0069] The seeds of Zhongmian 1279 and 10 other materials used as controls were removed, and the genomic DNA was extracted by SDS method, and diluted to a final concentration of 50 ng / μL.

[0070] 3. Amplification of SSR primers

[0071] Using the genomic DNA extracted above as a template, 182 pairs of primers (7 pairs of primers per chromosome on average) were selected for SSR PCR amplification for 26 chromosomes of upland cotton. The PCR reaction system was 10 μL, the amplification mixture system was shown in Table 1, and the PCR amplification program was shown in Table 2.

[0072] Table 1 PCR amplification reaction system

[0073] Reagent

volume

...

Embodiment 2

[0101]The hybrid cotton variety Zhongmian 1279 and other cotton varieties were detected according to the method in Example 1, and the results obtained were completely consistent with Example 1. It shows that the SSR primer pair provided by the present invention has good detection stability, and can effectively distinguish the hybrid cotton variety Zhongmian 1279 from other varieties.

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Abstract

The invention relates to the field of molecular detection, in particular to an SSR primer pair for identifying a hybrid cotton variety gossypium arboreum 1279 and a product and an identification method thereof. The SSR primer pair for identifying the hybrid cotton variety gossypium arboreum 1279 comprises any one or more of the following: NAU2836 upstream and downstream primers, BNL3255 upstream and downstream primers, NAU1233 upstream and downstream primers, NAU2671 upstream and downstream primers, NAU1070 upstream and downstream primers, CIR043 upstream and downstream primers, BNL1154 upstream and downstream primers and NAU3675 upstream and downstream primers. A total of 182 pairs of primers are designed to carry out PCR amplification on objects to be detected, the objects to be detectedinclude the hybrid cotton variety gossypium arboreum 1279 and 10 cotton varieties, thus different cotton varieties are compared, specific upstream and downstream primers for identifying the hybrid cotton variety gossypium arboreum 1279 are obtained through screening, the upstream and downstream primers are strong in specificity, and the hybrid cotton variety gossypium arboreum 1279 is well distinguished from other varieties.

Description

technical field [0001] The invention relates to the field of molecular detection, in particular to an SSR primer pair for identifying the hybrid cotton variety Zhongmian 1279, a product thereof, and an identification method. Background technique [0002] Utilization of heterosis is an effective means to increase cotton yield, improve fiber quality and enhance the adaptability of cotton varieties. Cotton hybrid varieties have been widely promoted in my country. However, cotton is a frequently cross-pollinated crop, and the field hybridization work is mainly through artificial pollination, which is time-consuming and labor-intensive, resulting in much higher seed production costs than conventional cotton. Driven by profit, there is often a phenomenon in the market where conventional cotton is used to impersonate or replace hybrids. Therefore, it is imperative to effectively identify hybrid cotton. [0003] At present, breeders often use traditional techniques and methods suc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 李威赵俊杰任中英杨代刚裴小雨刘艳改贺昆仑张飞马雄风周晓箭张文生
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI