Kit for detection of rhabdovirus in mandarin fish

A rhabdovirus and kit technology, applied in the field of biological diagnosis, can solve the problem of no kit for detecting mandarin fish rhabdovirus, and achieve reliable experimental results

Active Publication Date: 2022-03-25
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is currently no kit for the detection of rhabdovirus in mandarin fish

Method used

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  • Kit for detection of rhabdovirus in mandarin fish
  • Kit for detection of rhabdovirus in mandarin fish
  • Kit for detection of rhabdovirus in mandarin fish

Examples

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Embodiment 1

[0065] This embodiment provides a kit for detecting mandarin fish rhabdovirus, which includes sample diluent, blocking solution, washing solution, stop solution, negative control substance, positive control substance, The avidin was labeled with horseradish peroxidase, a capture antibody and a detection antibody. The capture antibody was 4H81F11 antibody, and the detection antibody was biotin-labeled 4E121F3. The capture antibody was coated in an Elisa well plate.

[0066] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should The technical solutions of the present invention may be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the pres...

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Abstract

The invention discloses a kit for detecting mandarin fish rhabdovirus, which belongs to the technical field of biological diagnosis. The kit of the invention includes a capture antibody (4H81F11 antibody) and a detection antibody (biotin-labeled 4E121F3 antibody), the capture antibody and the detection antibody Both can recognize the SCRV virus well, and the 4H81F11 antibody and the 4E121F3 antibody have different epitopes. On this basis, a double-antibody sandwich ELISA method for detecting the SCRV virus was established.

Description

technical field [0001] The invention belongs to the technical field of biological diagnosis, and in particular relates to a kit for detecting mandarin fish rhabdovirus. Background technique [0002] Mandarin fish rhabdovirus disease is caused by mandarin fish rhabdovirus (Siniperca chuatsi rhabdovirus, SCRV) and has a high-risk effect on mandarin fish (Siniperca chuatsi). The rapid onset and high mortality of mandarin fish rhabdovirus disease has seriously affected the mandarin fish aquaculture industry in my country. The mandarin fish rhabdovirus is one of the rhabdoviruses, a class of enveloped and unsegmented single-stranded negative-stranded RNA viruses. At present, the International Committee on Taxonomy of Viruses (ICTV) classifies rhabdoviruses into 18 genera, but some have not yet been classified [1]. The first strain of fish rhabdovirus was isolated from sick rainbow trout by Jensen et al. in Denmark in 1962 [2]. There are more than 20 kinds of fish rhabdoviruses ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20G01N33/53
Inventor 付小哲李宁求王璐瑶林强梁红茹刘礼辉牛银杰
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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