Fermented feed for improving growth performance of back-up dairy cattle and relieving heat stress, and preparation method and application thereof
A technology for fermented feed and back-up dairy cows, applied in applications, animal feed, animal feed, etc., can solve the problems of reduced absorption and utilization of nutrients, decreased feed intake of livestock and poultry, and accelerated respiratory rate, etc., to achieve increased crude fat content, The effect of increasing ash content and alleviating heat stress
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Embodiment 1
[0039] Embodiment 1 fermented feed preparation, pH value and nutrient composition determination
[0040] Feed to be fermented amounts to 4000kg, takes by weighing 400kg corn meal, 900kg oat hay, 2700kg herbal tea dregs.
[0041] According to the mass ratio of the feed to be fermented and the amount of molasses, weigh 80 kg of molasses and dissolve it in warm water at 37° C. to prepare molasses water with a final solubility of 2 to 3%.
[0042] According to the mass ratio of the feed to be fermented to yeast, Bacillus subtilis, Lactobacillus plantarum and Enterococcus faecalis, take yeast, Bacillus subtilis, Lactobacillus plantarum and Enterococcus faecalis powder respectively 1.2kg, 1.2kg, 1.2kg and 0.8 kg was dissolved in 2-3% molasses water, and placed in a 37°C incubator for 2 hours to revive the flora to obtain lysed molasses water. The total number of inoculated colonies of compound probiotics was (1-3)×10 8 CFU / g.
[0043] Take an appropriate amount of warm water at 37...
Embodiment 2
[0056] Example 2 Effect of fermented feed on the growth performance of back-up dairy cows
[0057] In the experiment, 90 healthy back-up dairy cows with similar age (7-8 months), feed intake and body weight were selected and randomly divided into 3 groups, 30 cows in each group, divided into control group, test group A and test group B. The control group was fed the total mixed diet (for the total mixed diet, please refer to the literature "Effects of different feeding methods on milk production performance, nutrient digestion and metabolism of southern dairy cows (Doctoral dissertation). Zhang Shirui, Yi Xuewu, Xiao Bingnan, & Liu Hailin. (2008). "), test groups A and B replaced 5% and 10% of the total mixed diet with the prepared fermented feed respectively. The test time was 40 days in total, the first 7 days were the pre-feeding period, and the formal test was 33 days.
[0058] Growth performance indicators include average daily feed intake, body length, body oblique leng...
Embodiment 3
[0066] Example 3 Effects of fermented feed on the ability of back-up dairy cows to relieve heat stress
[0067] On the last day of the experiment, 15 back-up dairy cows were randomly selected from the three groups, blood was collected by tailbone vein puncture, centrifuged at 4°C and 2500×g for 15 minutes, serum was collected, and stored at -20°C for detection of heat stress proteins, Lactate dehydrogenase, cortisol, creatine kinase, and alanine aminotransferase. The determination of the above indicators was provided by the test kit of Nanjing Jiancheng Bioengineering Research Institute.
[0068] It can be seen from Table 3 that the levels of heat stress proteins, lactate dehydrogenase, cortisol, creatine kinase and alanine aminotransferase in test group A and group B were significantly lower than those in the control group (p<0.05), and in test A Except that the content of alanine aminotransferase was not significantly different between group B and group B, the other four in...
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