Method for preservation, purification and rejuvenation of germplasm of trapa acornis nakano

Abstract

The invention discloses a method for preservation, purification and rejuvenation of germplasm of trapa acornis nakano, and belongs to the technical field of germplasm preservation. The method comprises the steps of (1) constructing a germplasm preservation pool, a germplasm identification pool, a germplasm rejuvenation pool and a cultivation seed growth pool; (2) collecting old ripened seeding water chestnuts with the characteristics of a trapa acornis nakano variety; (3) carrying out germination acceleration and seedling raising on collected and preserved water chestnut seeds, transplanting the water chestnut seeds into the germplasm identification pool for culturing, and screening out the water chestnut seeds which are in accord with the appearance characteristics of trapa acornis nakano; (4) rejuvenating the seeding water chestnuts of trapa acornis nakano; (5) raising the purified and rejuvenated original seedlings, transplanting the seedlings into the cultivation seed growth pool for culturing, screening the seedlings, and reserving seeds to serve as purified and rejuvenated cultivation seeds. The method for preservation, purification and rejuvenation of the germplasm of trapaacornis nakano standardizes the collection, identification and seed preservation modes and methods for trapa acornis nakano resources, and the original seed properties of trapa acornis nakano can be effectively preserved. Through production of the cultivation seeds, the purity of seeds used for production of trapa acornis nakano can be effectively ensured.

Description

Technical field [0001] The invention relates to the technical field of germplasm preservation, in particular to a method for germplasm preservation and purification and rejuvenation of Nanhu water chestnut. Background technique [0002] Nanhu water chestnut is a non-horned water chestnut, also known as wonton water chestnut, round water chestnut, and monk water chestnut. It is named after the main producing areas in Jiaxing South Lake and the surrounding waters in its history. Its skin is emerald green, with smooth ends and thin skin. The meat is tender, juicy, sweet and crisp, and fragrant than other varieties. It is often used as a special fruit for fresh food and is a treasure of Lingzhong. [0003] Nanhu water chestnut has a long history of cultivation in Jiaxing. According to records, the angleless carbonized water chestnut unearthed at the Majiabang cultural site in Jiaxing has a history of more than 6,000 years. Nanhu Ling, as a local specialty product of Jiaxing, has a str...

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Problems solved by technology

However, after the cultivation of water chestnut, farmers did not deal with the remains of water chestnut plants in time, and allowed the plant waste to rot freely on the river course, which had a great impact on water quality in a certain period of time. Restricted, resulting in the continuous reduction of Nanhu water chestnut planting area

[0005] With the decline of the planting area of ​​Nanhu water chestnut and the departure of some traditional growers, the preservation of the germplasm of Nanhu water chestnut has not been carried out well. The Nanhu water chestnut purchased on the market has long horns and other phenomena. risk of further loss

[0006] In addition, the limited cultivation of Waihenanhu water chestnut and the poor commerciality of Nanhu water chestnut and the poorer planting efficiency than other water chestnut varieties after cultivation in Nanhu water chestnut, led some farmers to prefer to plant red water chestnut, two-cornered green water chestnut and other varieties. With the planting of other water chestnut varieties, Nanhu water chestnut germplasm is affected by its biological characteristics, and different varieties cannot be well isolated, resulting in Nanhu water chestnut germplasm resources being polluted by other varieties of pollen, resulting in variety degradation

However, farmers are unable to purify and rejuvenate Nanhu water chestnut varieties during the cultivation process, which ultimately makes it difficult to buy pure Nanhu water chestnut products in the current market.

[0007] To sum up, Nanhu water chestnut, as the city card of Jiaxing City and the characteristic agricultural products beside the Nanhu Red Boat, has the risk of no land to grow and loss of germplasm resources

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