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Method for preventing and treating tobacco virus disease

A technology of tobacco virus and tobacco mosaic virus, applied in the fields of tobacco cultivation, botany equipment and methods, applications, etc., can solve problems such as outdated processing methods, limited planting yield, and failure to meet market demand, so as to improve yield and quality, The effect of increasing the total value, significant ecological benefits and economic benefits

Inactive Publication Date: 2019-07-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Comfrey extract is rich in shikonin, suitable for all skin types, and has the effects of anti-inflammation, curing blood, promoting skin regeneration, and cell metabolism; it can quickly penetrate into the skin, promote wound healing, and remove acne and acne marks. In recent years, the use of comfrey in disease treatment and pharmaceuticals has been increasing due to the effect of diluting pigmentation and scars. However, the traditional planting yield is limited, and the processing method is relatively backward, which cannot meet the market demand.

Method used

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  • Method for preventing and treating tobacco virus disease
  • Method for preventing and treating tobacco virus disease
  • Method for preventing and treating tobacco virus disease

Examples

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Effect test

Embodiment 1

[0044] Example 1 A method for preventing and treating tobacco virus disease

[0045] Tobacco and comfrey are intercropped and planted in fields with a high incidence of virus diseases. Real-time fluorescent quantitative PCR (realtime fluoresce quantitative polymerase chain reaction, q-PCR) technology was used to detect the content of TMV, CMV, and PVY in tobacco plants. Compared with the control group (non-intercropping, tobacco monocropping), the effect of comfrey root exudates on the virus in tobacco soil was analyzed.

[0046] 1. Experimental materials

[0047] Common tobacco (tobacco) variety K326 is provided by our laboratory. Lithospermumerythrorhizon, a plant of the family Comfrey. It was purchased from Luyong Seedling Co., Ltd., and the standard for selecting materials refers to "Chinese Medicine Dictionary" (Edited by Jiangsu New Medical College, 1975). The healthy tobacco seeds and comfrey seeds were sown in healthy substrates (tested, no virus). Water regularly and wa...

Embodiment 2

[0122] Tobacco and comfrey seedlings are transplanted into the field at the end of February each year. The variety of tobacco is Cuibi No.1. Plant the tobacco in rows, planting 2 rows of comfrey for every 2 rows of tobacco. The spacing (row spacing) between two adjacent rows of tobacco is 0.6m, and the spacing (plant spacing) between two adjacent tobacco plants in each row of tobacco is 1.0m. The distance between each row of comfrey and the adjacent row of tobacco is 0.7m. The distance between two adjacent rows of comfrey is 26cm, and the distance between two adjacent comfrey plants in each row is 26cm. No pesticides are applied during tobacco plant growth, and the rest of the operations are equivalent to field tobacco leaf production.

[0123] No pesticides are applied during the growth of tobacco plants. The remaining operations are equivalent to field tobacco production. This embodiment can simultaneously and effectively kill the TMV, CMV and PVY viruses in the soil of th...

Embodiment 3

[0125] Tobacco and comfrey seedlings are transplanted into the field at the end of February each year. The variety of tobacco is Honghua Dajinyuan. Plant the tobacco in rows, planting 2 rows of comfrey for every 2 rows of tobacco. The spacing (row spacing) between two adjacent rows of tobacco is 0.6m, and the spacing (plant spacing) between two adjacent tobacco plants in each row of tobacco is 0.5m. The distance between each row of comfrey and the adjacent row of tobacco is 0.5m. The distance between two adjacent rows of comfrey is 28cm, and the distance between two adjacent comfrey plants in each row is 28cm. No pesticides are applied during tobacco plant growth, and the rest of the operations are equivalent to field tobacco leaf production.

[0126] No pesticides are applied during the growth of tobacco plants. The remaining operations are equivalent to field tobacco production. This embodiment can simultaneously and effectively kill the TMV, CMV and PVY viruses in the soi...

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Abstract

The invention discloses a method for preventing and treating a tobacco virus disease. The method for preventing and treating the tobacco virus disease has the advantages that lithospermum erythrorhizon and nicotiana tabacum which have strong stress resistance, a good water and soil conservation effect and obvious economic benefits are selected for intercropping through a large amount of screening,and the beneficial allelopathy of the lithospermum erythrorhizon and nicotiana tabacum is utilized to effectively reduce diseases and insect pests, thereby being capable of not only improving the physicochemical property of soil and adjusting the soil fertility but also weakening the continuous cropping barrier; the interdependent relation among illumination, rainwater, saving lands and plants isfully utilized so that the incidence rate and harm degree of tobacco TMV, CMV and PVY diseases can be significantly reduced, the yield and quality of tobaccos are improved, and the tobaccos are ecological, safe, non-toxic and free of pollution; in addition, during planting, a bio-organic fertilizer is mainly used, intercropping of the lithospermum erythrorhizon and nicotiana tabacum can obtain the marginal effect, the land can be fully and reasonably utilized, the yield and the total value of crops are increased, the income of peasants is increased, and ecological and economic benefits are significant; the method is simple in operation, economical and practical, and has better popularization and application prospects.

Description

Technical field [0001] The invention belongs to the technical field of tobacco cultivation. Specifically, it relates to a method for controlling tobacco virus diseases. Background technique [0002] People have a long history of research on the virus carried in the soil. In 1980, people found a variety of human pathogenic viruses in soil and wastewater, and it was determined that soil and wastewater were the main sources of infection of human pathogenic viruses (Lin Changsheng, 1980). In 1995, researchers explored the retention of Heliothis armigera nuclear polyhedrosis virus (HaNPv) in soil and the effect of soil pH on the biological activity of HaNPv (Zhang Zhongxin, 1995). In 2006, Zhao Bingzi (2006) used MS2 and -174 as indicator viruses to study the main factors affecting the migration of viruses in soil. Wang Qiuying et al. (2007) explored the adsorption behavior of soil to viruses and its purification effect in the environment, and found that red clay has the strongest ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/45A01G13/00
CPCA01G13/00A01G22/45
Inventor 阮小蕾
Owner SOUTH CHINA AGRI UNIV
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