IL-6/PCT combined detection time-resolved detection kit and method

Abstract

The invention relates to a IL-6/PCT combined detection time-resolved fluorescence immunochromatography quantitative detection kit and a detection method, which utilize the sensitivity of a time-resolved fluorescence immunochromatography technology and rare earth vanadate nano fluorescence labeling materials and combine a dry-type immunofluorescence analyzer to realize high sensitivity, accurate quantification, simplicity and convenience and rapidness. The detection kit can accurately and quantitatively detect the contents of interleukin 6 and procalcitonin in human serum, plasma and whole blood samples, the samples can be processed by the blood filtering membrane sample pad without pretreatment and dilution by diluent, the fluorescence interference of the samples can be avoided by using atime-resolved fluorescence microsphere detection technology, the rare earth vanadate nano material has the advantages of low background, strong fluorescence signal, high signal-to-noise ratio and thelike, the rare earth vanadate nano particles are connected with the antibody through a covalent bond, and the labeled product is stable, so that the detection kit has the characteristics of wide detection range, high sensitivity, high accuracy, quick and simple detection and the like.

Description

[0001] (1) Technical field [0002] The invention relates to an IL-6 / PCT combined detection time-resolved fluorescence detection kit and method. [0003] (2) Background technology [0004] Interleukin 6 (IL-6) is a cytokine, a type of interleukin, that can stimulate the proliferation, differentiation and function of cells involved in the immune response. IL-6 and its receptors are related to inflammatory diseases. It is a multifunctional inflammatory cytokine and plays an important role in inflammatory response. Elevated levels of IL-6 can be used as an early indicator of inflammatory response and can be used to assess the severity of systemic inflammatory response syndrome (SIRS). When the inflammatory response occurs, the serum concentration of IL-6 rises earlier than other biomarkers, and induces the production of PCT and CRP after the concentration rises. The concentration of IL-6 in the patient's serum rises rapidly in the early stage of infection and reaches the peak wi...

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Problems solved by technology

[0007] In the prior art, the detection of inflammatory infection and sepsis only detects a single inflammatory index. In addition, there are few test strips for IL-6 detection, and the methods for measuring IL-6 / PCT mainly include immunoturbidimetry, fluorescent immunolayer analysis method, colloidal gold method, etc.

Among them, the colloidal gold method has the advantages of simple and fast operation, but low sensitivity and inaccurate quantification; immunoturbidimetric method is more sensitive and accurate, and can be applied to automatic biochemical analyzers, but requires instruments and takes a long time, and is suitable for processing a large number of samples. It cannot meet the purpose of rapid detection; the immunofluorescence method is to covalently bind the IL-6 / PCT antibody to the surface active group of the fluorescent microsphere, and judge the result by whether the detection line produces fluorescence after excitation, which is fast, convenient, accurate and quantitative. With the advantages of high sensitivity and stable markers, it is widely used in the field of medical immunoassay

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