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Application of dendrobium polysaccharide in preparation of drugs for prevention and treatment of reproductive injury caused by arsenic poisoning

A technology for dendrobium polysaccharide and arsenic poisoning, which is applied in the directions of drug combination, antidote, pharmaceutical formulation, etc., to achieve the effects of no toxic side effects, low cost, and wide source of raw materials

Inactive Publication Date: 2019-07-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research and application of Dendrobium officinale polysaccharides for the prevention and treatment of reproductive damage caused by arsenic exposure.

Method used

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  • Application of dendrobium polysaccharide in preparation of drugs for prevention and treatment of reproductive injury caused by arsenic poisoning
  • Application of dendrobium polysaccharide in preparation of drugs for prevention and treatment of reproductive injury caused by arsenic poisoning
  • Application of dendrobium polysaccharide in preparation of drugs for prevention and treatment of reproductive injury caused by arsenic poisoning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The extraction of embodiment 1 Dendrobium officinale polysaccharide

[0044] 1. Extraction of Dendrobium polysaccharides

[0045] Weigh 4g of Dendrobium officinale powder (purchased from the Crop Research Institute of the Guangdong Academy of Agricultural Sciences), add 10 times the volume of distilled water, fully dissolve, and bathe in a water bath at 90°C for 100 minutes, stirring once every 30 minutes during this period, and filter with gauze to obtain filtrate. Repeat the above process 3 times, combine the filtrates, concentrate with a freeze dryer, add 4 times the volume of absolute ethanol, precipitate with alcohol at 4°C for 12 hours and centrifuge to obtain a precipitate, then wash the precipitate with 80% ethanol for 2 to 3 times, at 40°C Dried to obtain the crude polysaccharide powder of Dendrobium officinale.

[0046] 2. Determination of polysaccharide content

[0047] Weigh 100mg of anhydrous glucose, place it in a dry 100mL volumetric flask, add distill...

Embodiment 2

[0049] Embodiment 2 animal experiments

[0050] 1. Solution preparation

[0051] (1)As 2 o 3 Preparation of solution

[0052] Weigh As 2 o 3 Put 200mg of powder in a 50mL beaker, add 2mL of 2.5mol / L NaOH solution to dissolve it completely, transfer it to a 100mL volumetric flask, distill the volume to 100mL with distilled water, shake well to get As 2 o 3 solution.

[0053] (2) Preparation of Dendrobium candidum polysaccharide solution

[0054] Weigh 625.55mg of Dendrobium officinale crude polysaccharide powder prepared in Example 1 in a 50mL beaker, add 20mL of distilled water to dissolve it completely, transfer it to a 50mL volumetric flask, distill it to 50mL with distilled water, and shake it up to obtain 10mg / mL of Dendrobium officinale crude polysaccharide solution.

[0055] (3) Preparation of HTF solution (human fallopian tube culture fluid)

[0056] Prepare the HTF solution according to the formula in Table 1.

[0057] Table 1 HTF solution formula

[0058]...

Embodiment 3

[0071] The detection of embodiment 3 mouse sperm quantity and vitality

[0072] 1. Detection of sperm count

[0073] At the end of the experiment period, the male rats were killed by cervical dislocation, and the tail of the epididymis was dissected and quickly cut to make a sperm suspension. Take 10 μL of the sperm suspension and suck it into the red blood cell counting board, and count the number of sperm in 5 small squares according to the red blood cell counting method. , to calculate the number of spermatozoa per milliliter of sperm suspension.

[0074] Table 2 The effect of DOP on the number of sperm in mice (unit: ×10 6 each / mL)

[0075]

[0076] The data is n=10. "*" indicates a significant difference (P<0.05) compared with the blank control group at the same treatment time; "#" indicates a significant difference (P<0.05) compared with the arsenic exposure group at the same treatment time.

[0077] The results are shown in Table 2 and figure 1 As shown, compa...

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Abstract

The invention provides an application of dendrobium polysaccharide in the preparation of drugs for prevention and treatment of reproductive injury caused by arsenic poisoning. The dendrobium polysaccharide has obvious therapeutic effect on reproductive injury induced by arsenic exposure in male rats, which can significantly increase the number and motility of sperms, increase the incidence of sperm acrosome reaction and decrease the rate of sperm deformity; the SOD activity, GPx activity, CAT activity and GSH content in testicular tissue are also significantly increased, while MDA content is also significantly decreased; the dendrobium polysaccharide has rarely toxic and side effects and no adverse effect on normal growth and physiological state. The dendrobium polysaccharide with wide source of raw materials is simple in extraction and purification method, high in sugar content and suitable for large-scale production and promotion and has a good prospect in the preparation and / or treatment of reproductive injury drugs caused by arsenic poisoning and clinical application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the application of dendrobium polysaccharides in the preparation of medicines for preventing and / or treating reproductive damage caused by arsenic poisoning. Background technique [0002] Arsenic is a metalloid element that exists in nature, mainly in the form of arsenic trioxide (As 2 o 3 ), arsenic disulfide (realgar), arsenic trisulfide (orpiment), and sulfur arsenide iron compounds. For humans, arsenic is a toxic and carcinogenic substance present in the environment. Long-term exposure to high arsenic-containing environments can cause acute and chronic arsenic poisoning, teratogenic, mutagenic and carcinogenic effects, and damage the human heart, liver, kidney, skin, reproductive system and other organs and tissues. In 2003, the World Health Organization stated that more than 100 million people worldwide were at risk of exposure to high levels of arsenic. The World...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P15/08A61P39/02A61P39/06
CPCA61K31/715A61K36/8984
Inventor 芦春斌朱倍倍李春梦姚炜炜仇平乐
Owner JINAN UNIVERSITY
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