Microfluidic chip
A microfluidic chip and microchannel technology, which is applied in the field of microfluidics, can solve the problems of microchannel blockage, the reduction of the number of freeze-dried reagents, and the inability to add sample liquid, so as to avoid blockage and reduce the loss rate.
Pending Publication Date: 2019-07-09
融智生物科技(青岛)有限公司
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Problems solved by technology
However, the inventors found that after the reagent was added to the reaction chamber, the liquid reagent would diffuse along the microchannel communicating with the reaction chamber, resulting in a reduction in the amount of freeze-dried reagents remaining in the reaction chamber, and more seriously, the microchannel would be blocked. The sample solution cannot be added to the reaction chamber
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[0026] In order to illustrate the present invention more clearly, the present invention will be further described below in conjunction with preferred embodiments and accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not limit the protection scope of the present invention.
[0027] Such as Figure 1-4 As shown, the embodiment of the present invention provides a microfluidic chip, the chip includes a cover 1 and a substrate 2, the substrate 2 is provided with at least one reaction chamber 21, and the sample injection chambers communicated with the reaction chamber 21 through microchannels respectively. port 22 and air outlet 23. The cover sheet 1 is fixedly bonded to the surface of the substrate 2 to seal and seal the reaction chamber 21 and the microchannel. The reaction chamber 21 and the m...
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The invention discloses a microfluidic chip. The microfluidic chip comprises a substrate and a cover sheet; the substrate is provided with at least one reaction chamber, and a sample inlet and a gas outlet respectively communicating with the reaction chamber through a microchannel; the cover sheet is fixedly combined with the surface of the substrate for sealing the reaction chamber and the microchannel; and the side wall of the reaction chamber has a stepped structure, and the area of the bottom surface of the reaction chamber is smaller than the area of an opening located in the top surface.According to the microfluidic chip provided by the invention, the stepped structure is arranged on the side wall of the reaction chamber of the microfluidic chip to block a reagent from dispersing and entering the microchannel during sample application and lyophilization, so that clogging of the microchannel after lyophilization is avoided, and smooth and accurate sample adding operation in follow-up experiments is ensured.
Description
technical field [0001] The invention relates to the field of microfluidic technology. More specifically, it relates to a microfluidic chip. Background technique [0002] Microfluidic chip is the main platform for the realization of microfluidic technology. Microfluidic chip refers to the integration of basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of chemistry and biology into a small chip, and a network of microchannels is formed, with controllable fluid running through the entire chip. System, used to realize various functions of conventional chemical or biological laboratory. The microfluidic chip can quickly and accurately divide the sample into several independent units, and perform multi-step parallel reactions, with low cost, small volume and high throughput. [0003] There are two main types of common PCR microfluidic chips: micro-reaction chamber type and continuous flow type. Micro-reaction chamber ...
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IPC IPC(8): B01L3/00C12M1/00B01L7/00
CPCB01L3/5027B01L7/52B01L2200/025B01L2300/0829B01L2300/0861B01L2300/1894
Inventor 陈娇冯政德潘国龙李运涛周晓光
Owner 融智生物科技(青岛)有限公司