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Ussuriwawe's Rapid Propagation Method

A technology of Ussuri and Wawe, which is applied in the field of rapid propagation of Ussuri and Wawe, can solve problems such as difficult rooting, long cultivation cycle, and restricting the development of propagation methods, so as to save time, save time and economic costs, and realize The effect of rapid reproduction

Active Publication Date: 2022-01-04
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of the Ussuriwawee tissue culture method, there are problems such as long cultivation period, difficult spore germination, and difficult rooting, etc. These problems restrict the development of Ussuriwawee propagation method

Method used

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  • Ussuriwawe's Rapid Propagation Method
  • Ussuriwawe's Rapid Propagation Method
  • Ussuriwawe's Rapid Propagation Method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Obtaining explants: Take the leaves of Ussurivawe with yellow-green sporangia, gently flick off the impurities, put them in an envelope, dry them in a cool and ventilated place for 2 days, and store them in a refrigerator at 4°C for later use. The whole process keeps the sporangia intact;

[0042] (2) Disinfection of explants: soak the explants obtained in step (1) with sterile water for 24 hours, sterilize them with 75% ethanol for 30 seconds, rinse them with sterile water for 3 times, and then wash them with 0.1% HgCl 2 After the solution is sterilized for 8 minutes, rinse it with sterile water for 3 times before use, without breaking the sporangia during the whole process;

[0043] (3) Induce germination: Scrape off the sporangia of the Ussurivawe leaves with sporangia after disinfection in step (2), scrape off the sporangia with a clean blade, place them in sterile water, and dip a clean brush in sterile water to gently Points are cultured on the surface of the...

Embodiment 2

[0053] (1) Obtaining explants: Take the leaves of Ussurivawe with yellow-green sporangia, gently flick off the impurities, put them in an envelope, dry them in a cool and ventilated place for 2 days, and store them in a refrigerator at 4°C for later use. The whole process keeps the sporangia intact;

[0054] (2) Disinfection of explants: soak the explants obtained in step (1) with sterile water for 20 hours, sterilize them with 75% ethanol for 40 seconds, rinse them with sterile water for 3 times, and then use 0.1% HgCl 2 After the solution is sterilized for 8 minutes, rinse it with sterile water for 3 times before use, without breaking the sporangia during the whole process;

[0055] (3) Induce germination: Scrape off the sporangia of the Ussurivawe leaves with sporangia after disinfection in step (2), scrape off the sporangia with a clean blade, place them in sterile water, and dip a clean brush in sterile water to gently Points are cultured on the surface of the induction ...

Embodiment 3

[0065] (1) Obtaining explants: Take the leaves of Ussurivawe with yellow-green sporangia, gently flick off the impurities, put them in an envelope, dry them in a cool and ventilated place for 2 days, and store them in a refrigerator at 4°C for later use. The whole process keeps the sporangia intact;

[0066] (2) Disinfection of explants: soak the explants obtained in step (1) with sterile water for 30 hours, sterilize them with 75% ethanol for 20 seconds, rinse them with sterile water for 5 times, and then use 0.1% HgCl 2 After the solution is sterilized for 8 minutes, rinse it with sterile water for 5 times before use, without breaking the sporangia during the whole process;

[0067] (3) Induce germination: Scrape off the sporangia of the Ussurivawe leaves with sporangia after disinfection in step (2), scrape off the sporangia with a clean blade, place them in sterile water, and dip a clean brush in sterile water to gently Points are cultured on the surface of the induction ...

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Abstract

The invention discloses a rapid propagation method of Ussurivavir, through a series of steps of obtaining explants, disinfecting the explants, inducing germination, and inducing rooting to obtain the isolated regenerated plants of Ussurivavir, realizing the Ussuriwawee reproduces rapidly, has the advantages of short cultivation period, high spore germination rate, high rooting rate, and low production cost, which is conducive to the large-scale production of Ussuriwawee.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a rapid propagation method of Usurivavir. Background technique [0002] Ussurivave is a plant of the genus Vavee in the family Pteridaceae, which has strong anti-inflammatory, analgesic, antibacterial, anti-oxidative, liver-protecting, and cough-relieving effects. Ussuriwawe mainly comes from the wild, but there are very few wild resources, which cannot meet the growing needs of human beings. Only by solving the technical difficulties of rapid reproduction can the effective protection and sustainable use of resources be realized. However, there is no report on the method of artificially cultivating Ussurivawe at present. [0003] The common propagation methods of ferns include spore propagation, branch propagation and tissue culture. The competitiveness and tolerance of spores in the natural environment are poor, and the germination rate is low, so they cannot be widely u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王丽红张春萌陈尚君崔红赵龙张义秀
Owner JIAMUSI UNIVERSITY
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