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Salt-tolerance growth-promoting bacterium strain Y4 and application thereof

A growth-promoting bacteria and salt-tolerant technology, applied in the field of microorganisms, to achieve the effects of promoting dry matter accumulation, promoting root tip count, and improving salt tolerance

Active Publication Date: 2019-07-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research work on PGPR improving saline soil and promoting plant salt tolerance is still in its infancy, mainly focusing on Pseudomonas spp., Bacillus spp., etc., and their role in adjusting plant Some mechanistic discussions have been made on the balance of endogenous hormones, ion homeostasis, anti-oxidation and photosynthesis
However, there are few introductions about the salt-tolerant rhizosphere growth-promoting bacteria of tomato

Method used

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  • Salt-tolerance growth-promoting bacterium strain Y4 and application thereof
  • Salt-tolerance growth-promoting bacterium strain Y4 and application thereof
  • Salt-tolerance growth-promoting bacterium strain Y4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation, screening and identification of salt-tolerant growth-promoting bacterial strain Y4

[0016] Weigh 2.0g of Yancheng rapeseed rhizosphere soil sample (with plant roots) and place it in a conical flask filled with 100mL of double-distilled water, seal it with a parafilm, and shake it in a shaker at 200r / min at 30°C for 24h to obtain soil suspension. Take 1mL of soil suspension and transfer it to 100mL LB liquid medium containing 2% NaCl, culture at 30°C at 200r / min for 24h, then transfer again to 4% NaCl containing Cultivate in LB medium for 24h, shake culture at 30°C for 24h, then transfer to LB medium with 6% NaCl content, shake culture at 30°C for 24h, and incubate the obtained bacterial culture solution for 10 6 Dilute and spread, and pick a single colony that grows well, that is, the salt-tolerant growth-promoting bacteria strain Y4 is screened.

Embodiment 2

[0017] Example 2 Identification of salt-tolerant growth-promoting bacterial strain Y4

[0018] 1. Bacterial DNA Extraction

[0019] The bacteria isolated and screened in Example 1 were cultivated to the logarithmic phase, and 1mL of bacterial liquid was drawn to a 1.5mL centrifuge tube, first inactivated in a 30min boiling water bath, and then placed in a centrifuge to centrifuge at 1000rpm for 5min, and the resulting supernatant was And for bacterial DNA extraction.

[0020] 2. PCR amplification of strain 16s rDNA

[0021] The bacterial DNA was used as a template, and the amplification primers used bacterial universal primers, namely: forward primer U8-27(F) 5'-AGAGTTTGATCCTGGCTCA-3'; reverse primer L1494-1514(R) 5'-GGTTACCTTGTTACGACTT-3 '. 50 μL PCR amplification system: 21 μL dd water, 1 μL each of forward and reverse primers, 25 μL R-Taq mix, 2 μL of the above DNA extract. Add each substance in the above system in order to a 0.5mL PCR tube, mix well, and centrifuge at ...

Embodiment 3

[0025] Example 3 Salt-tolerant growth-promoting test of tomato strain Y4

[0026] Preparation of tomato growth promoter: Y4 was inoculated in LB liquid medium, cultivated at 180rpm and 30°C until OD600 was 1 (ie 10 8 CFU / mL), to obtain the bacterial solution, centrifuge the bacterial solution at a speed of 6000rpm for 10min, discard the supernatant, then inject an equal volume of dd water, shake and resuspend, centrifuge at a speed of 6000rpm for 10min, discard the supernatant, repeat 2 After resuspension, the tomato growth promoter is obtained.

[0027]Experimental design: select plump tomato seeds of the same size and sterilize the surface with 5% NaClO solution for 10 minutes, rinse the seeds repeatedly with deionized water for several times, soak the seeds in deionized water for 24 hours, and then place them in a culture medium covered with moist gauze. Germinate on a dish at 27°C for 2 days. The seeds with the same bud length were selected and sown in plastic pots (pot ...

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Abstract

The invention relates to a salt-tolerance growth-promoting bacterium strain Y4. The salt-tolerance growth-promoting bacterium strain Y4 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.17707, the preservation date is 5 May 2019, and the 16S rDNA nucleotide sequence of the salt-tolerance growth-promoting bacterium strain Y4 is as shownin SEQID NO.1. The strain Y4 disclosed by the invention can significantly improve the salt-tolerance growth-promoting capacity of tomatoes under salt stress, the strain is prepared into a tomato growth promoting agent, and then the tomato growth promoting agent is filled into tomato seedling rhizosphere soil, so that plant type, stem height and stem thickness of the tomatoes can be obviously enlarged, the dry matter accumulation of tomato seedlings can be significantly promoted, the strain Y4 has promoting effects on root length, surface area, root volume and the number of root tips of the tomato seedlings, a salt injury phenomenon is retarded, the growth of the tomato seedlings is promoted, and increase of the salt tolerance of the tomatoes is facilitated.

Description

technical field [0001] The invention relates to a salt-tolerant growth-promoting bacterial strain Y4 and an application thereof, belonging to the technical field of microorganisms. Background technique [0002] Tomato is the most planted Solanaceae vegetable crop after potato in the world, and it is also an important model plant for fruit and Solanaceae (Salvalaggio et al., 2017; Zhang Tianpeng and Yang Xinghong, 2018). It is deeply loved and welcomed by the public. More than 3,000 tomato varieties have been reported worldwide, and global tomato production is facing severe challenges from biotic and abiotic stresses. Salinity is a major environmental stress in agriculture that inhibits crop growth and reduces food yields to limit the productivity of major food crops worldwide (Kaushal et al., 2016; Li Lulu et al., 2019). Tomatoes are moderately salt sensitive and are mainly grown in warm and arid regions of the world where soils tend to be relatively salinity. With the inc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01G22/05A01G13/10C12R1/01
CPCC12N1/20A01N63/00A01G22/05A01G13/10C12R2001/01C12N1/205
Inventor 郑青松兰汝佳陈军赵海燕邹明之魏龙
Owner NANJING AGRICULTURAL UNIVERSITY
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