SERS detection method for ochratoxin A

A technology of ochratoxin and detection method, which is applied in the field of detection of mycotoxins, and can solve problems such as time-consuming, inability to meet the needs of food quality and safety control, and inability to meet quick inspections

Active Publication Date: 2019-07-23
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current reports on SERS detection of OTA are mainly some newer detection methods based on aptamers, such as Galarreta et al. (B Galarreta. Microfluidic channel with embedded SERS 2D platform for the aptamer detection of ochratoxin A[J]. Chemistry, 2013,405(5):1613-1621) reported that the aptamer-based SERS sensor detects OTA, and the detection is 50nM (about 24μg/kg), but it cannot meet the needs of food quality and safety control
Gillibert et al. (R Gillibert.Surface enhanced Raman scattering sensor for highly sensitive and selctive detection of ochratoxin A[J].Analyst,2018,143(1):339-345) connected the aptamer to the biological signal reporter molecule and developed The detection of OTA by the aptamer-based SERS sensor has achieved high precision of picomolar concentration, but the functionalization of the substrate takes a long time and needs to be capped with 6-mercaptohexanol, which cannot m

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  • SERS detection method for ochratoxin A
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  • SERS detection method for ochratoxin A

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1) Synthesis of Au nanoparticles:

[0029] Au nanoparticles (AuNPs) solutions were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle solution with a particle size of approximately 55 nm.

[0030] 2) Preparation and characterization of TGA modified gold nanoparticles:

[0031] Take 40 mL of the above-mentioned gold nano-sol in a clean round bottom flask, add the TGA solution dropwise under 1000r / min magnetic stirring, and continue the reaction for 20 minutes to obtain TGA-modified gold nanoparticles. The synthesized TGA-modified gold nanoparticles were Infrared spectrum, EDS, SEM characterization.

[0032] Take the TGA-modified gold nanoparticles in step 2), concentrate and put them to...

Embodiment 2

[0037] 1) Synthesis of Au nanoparticles:

[0038] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle solution with a particle size of approximately 55 nm.

[0039] 2) Synthesis of TGA-modified gold nanoparticles and preparation of SERS substrate:

[0040] Take 40 mL of the above-mentioned gold nano solution in a clean round bottom flask, add 200 μL TGA solution dropwise under 1000 r / min magnetic stirring, and continue the reaction for 20 min to obtain a TGA-modified gold nano particle solution. Take 1.0 mL of the synthesized TGA-modified gold nanoparticle solution, centrifuge at 7000 r / min for 5 min, discard the supernatant, add 200 μL ultrapure water to wash once...

Embodiment 3

[0046] 1) Synthesis of Au nanoparticles:

[0047] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticles with a particle size of approximately 55nm.

[0048] 2) Synthesis of TGA-modified gold nanoparticles and preparation of SERS substrate:

[0049] Take 40 mL of the above-mentioned gold nano-sol in a clean round bottom flask, add 200 μL of TGA solution dropwise under 1000 r / min magnetic stirring, and continue the reaction for 20 min to obtain a TGA-modified gold nano-particle solution. Take 1.0 mL of the synthesized TGA-modified gold nanoparticle sol, centrifuge at 7000 r / min for 5 min, discard the supernatant, wash once with 200 μL ultrapure water, and concentrate ...

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Abstract

A SERS detection method for ochratoxin A involves the detection of mycotoxins. The method comprises the steps of preparing an Au nanoparticle sol by reducing chloroauric acid with sodium citrate, preparing TGA-modified gold nanoparticles, and performing quantitative detection in OTA. By taking the gold nanoparticles modified with thioglycolic acid as the SERS substrate, on the one hand, the surface energy of gold nanoparticles is reduced, the stability of gold nanoparticles is improved and the agglomeration of nanoparticles is prevented, and on the other hand, a hydrogen bond is formed by means of the free carboxyl in TGA molecules and carbonyl oxygen in OTA molecules, the OTA is pulled into the local electromagnetic field enhancement region of the gold nanoparticle, that is, the SERS hotspot region, and the Raman detection signal is enhanced. By using the linear relationship between the signal intensity and concentration of the characteristic Raman peak at 1265cm-1 of OTA, a simple,rapid and low-cost quantitative analysis method by directly using SERS characteristic peak of OTA is established, without uncomplicated specificity modification.

Description

technical field [0001] The invention relates to the detection of mycotoxins, in particular to a SERS detection method for ochratoxin A. Background technique [0002] Ochratoxin (Ochratoxin, OT) is a mycotoxin produced by fungi of the genus Herperus and Penicillium. There are mainly four types: A, B, C, and D. It is the most toxic in , second only to aflatoxin, and is most relevant to human health. OTA is ubiquitous in a variety of food commodities such as grains, wheat, barley, corn, coffee, and wine, and the pollution of corn and peanuts is particularly serious and common. OTA has nephrotoxicity, liver toxicity, immunotoxicity and mutagenic effects on humans and animals (J Gil-Serna.Revision of ochratoxin a production capacity by the main species of Aspergillus section Circumdati Aspergillus steynii revealed as the main risk of OTA contamination[J] .Food Control,2011,22(2):343-345; S Ozden.Occurrence of ochratoxin A in cereal-derived foodproducts commonly consumed in Turk...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 张芹贺路影陈艳红苏文金
Owner JIMEI UNIV
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