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Brevundimonas scallop capable of efficiently producing carotenoid and culture method and application thereof

A technology of brevundimonas and carotene, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effect of low cost, easy promotion and simple operation

Pending Publication Date: 2019-08-09
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, to date, the isolation of carotenoid-producing bacteria from marine animals has not been reported

Method used

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  • Brevundimonas scallop capable of efficiently producing carotenoid and culture method and application thereof
  • Brevundimonas scallop capable of efficiently producing carotenoid and culture method and application thereof
  • Brevundimonas scallop capable of efficiently producing carotenoid and culture method and application thereof

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Embodiment 1

[0026] Example 1: Isolation, identification, optimization of culture conditions and determination of carotenoid production efficiency of Brevundimonas scallop Zheng&Liu 501

[0027] In November 2016, 20-30 golden individuals were randomly selected from the 8-month-old Chlamys farreri cultured in the sea area of ​​Nan'ao Island, Shantou City, Guangdong Province. The average shell height and weight were 54.67±1.34mm and 42.77±4.12g, respectively; Dissect the scallops, take the intestinal tract, homogenate, dilute, and then conduct sterile culture. After culturing at room temperature 25±2°C for 72 hours, pick out the orange and red colonies for purification and identification; further optimize the culture conditions. Expand the culture for 72 hours; finally extract the carotenoids and measure their content. The specific steps are as follows:

[0028] 1. Isolation of bacteria. Use sterile PBS to homogenize the intestinal tract and dilute it in a gradient, then add 100 μL of each...

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Abstract

The invention provides a strain capable of producing carotenoid and being separated from seawater bivalve mollusc-luxury chlamys farreri intestinal tracts. The strain is named as Brevundimonas scallopZheng&Liu, is preserved in the China Center for Type Culture Collection, and the preservation number of the strain is CCTCC NO:M2019023. The strain has the advantages of being greatly suitable for the oligotrophic environment and efficiently producing carotenoid. The invention further relates to a culture method of the Brevundimonas scallop Zheng&Liu. The method specifically comprises the steps that the Brevundimonas scallop Zheng&Liu is cultured in a common LB culture medium for 3-5 d on the conditions that the PH value is 7-9, the inoculation amount is 0.05-0.5%, the rotating speed of a table is 100-200 r / min, and the temperature is 20-30 DEG C, and 3.92 mg / L-4.56 mg / L carotenoid can be produced, and no cell wall breaking or drying treatment needs to be performed in the carotenoid extracting process. The Brevundimonas scallop Zheng&Liu has the advantages of being high in production efficiency, low in cost, easy to operate and the like.

Description

technical field [0001] The invention belongs to the field of marine bacteria and their cultivation and application, and in particular relates to a strain of Brevundimonas scallop that efficiently produces carotenoids and its cultivation method and application. Background technique [0002] Carotenoids are a class of fat-soluble pigments that naturally exist in nature. They belong to terpenoids and contain a series of methyl branched chains and conjugated double bonds. Different color characteristics due to the properties of substituents. Since the study of carotenoids in the 19th century, nearly 1,000 kinds of carotenoids have been discovered, all of which have various biological functions. Neither humans nor animals can synthesize carotenoids themselves, and they are mainly absorbed through food intake. Carotenoids in nature are mainly synthesized by plants and microorganisms (microalgae, bacteria and fungi). [0003] Carotenoids have many beneficial biological activitie...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P23/00C12R1/01
CPCC12N1/20C12P23/00C12R2001/01C12N1/205
Inventor 郑怀平刘宏星江胜
Owner SHANTOU UNIV