A method for identifying the size of muscle fiber density of pigs and the primer pair used therefor

A technology for muscle fibers and determination methods, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The effect of muscle fiber density

Active Publication Date: 2022-04-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, muscle fiber density can only be measured after slaughter, making breeding difficult

Method used

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  • A method for identifying the size of muscle fiber density of pigs and the primer pair used therefor
  • A method for identifying the size of muscle fiber density of pigs and the primer pair used therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 identifies the muscle fiber density size of pig

[0040] 1. Determination of polymorphism site of pig SSC14g.85531863C>T

[0041] (1) Using two Beijing black pigs as experimental materials, the genomic DNA of the ear margin tissue was extracted respectively.

[0042] (2) Design and synthesis of primers

[0043] According to the sequence of the pig reference genome Sscrofa11.1, the following primers were designed and synthesized:

[0044] U (upstream primer): 5'-CTGCACCTGACTCTTTCCC-3' (shown in sequence 1);

[0045] D (downstream primer): 5'-GGTCCACAGATTCCCCTTCC-3' (shown in SEQ ID NO: 2).

[0046] (3) PCR amplification

[0047] The genomic DNA of the Beijing black pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain two PCR amplification products, which were named product 1 and product 2, respectively.

[0048] PCR amplification system: Genomic DNA 200ng, 10×PCR amplification buffer ...

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Abstract

The invention discloses a method for identifying the muscle fiber density of pigs and a pair of primers used therefor. A pair of primers for amplifying a DNA fragment containing the SSC14 g.85531863C>T polymorphic site; the SSC14 g.85531863C>T polymorphic site is from chromosome 14 of the pig reference genome Sscrofa11.1 from 5 The 85531863rd nucleotide from the 'end; the pig reference genome Sscrofa11.1 is the pig reference genome sequence updated in February 2017 in GenBank. The present invention can carry out early screening of pigs to be selected, effectively alleviates the problem of long time for selecting excellent breeding pigs in actual production, reduces breeding costs, and effectively reduces or increases the density of pig muscle fibers. The method has high accuracy, low detection cost, and It can realize automatic detection and has high practical application value in pig breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying the density of pig muscle fibers and a pair of primers used therefor. Background technique [0002] Pork quality is an important economic trait of pigs, and it is closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Therefore, the research on pork quality has become an important part in the fields of meat food processing and pig genetics and breeding. The muscle fiber density, which is the main factor affecting pork quality, has also attracted more and more attention from researchers and breeders, and has become a key component of pig breeding programs. part. [0003] However, muscle fiber density can only be measured after slaughter, making breeding difficult. Molecular breeding can carry out breeding work by screening molecular markers associated with traits to avoid slaughter. Therefore, it is ver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 张龙超王立贤王立刚刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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