Method for identifying diameters of muscle fibers of pig and primer pair used by method

A primer pair, muscle fiber technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficulties in breeding work, achieve high practical application value, low testing costs, and reduce breeding. effect of spending

Active Publication Date: 2019-08-23
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the muscle fiber diameter can only be measured after slaughter, making breeding difficult

Method used

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  • Method for identifying diameters of muscle fibers of pig and primer pair used by method
  • Method for identifying diameters of muscle fibers of pig and primer pair used by method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 identifies the muscle fiber diameter size of pig

[0040] 1. Determination of SSC4g.69991162A>G polymorphism in pigs

[0041] (1) Using two Beijing black pigs as experimental materials, the genomic DNA of the ear margin tissue was extracted respectively.

[0042] (2) Design and synthesis of primers

[0043] According to the sequence of pig reference genome 11.1, the following primers were designed and synthesized:

[0044] U (upstream primer): 5'-CAGCTCCATATTTCCTAAGTC-3' (shown in sequence 1);

[0045] D (downstream primer): 5'-GCAGAAGTTTCATAAGGCACATGAA-3' (shown in SEQ ID NO: 2).

[0046] (3) PCR amplification

[0047] The genomic DNA of the Beijing black pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain two PCR amplification products, which were named product 1 and product 2, respectively.

[0048] PCR amplification system: Genomic DNA 200ng, 10×PCR amplification buffer 5μl, dNT...

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Abstract

The invention discloses a method for identifying diameters of muscle fibers of a pig and a primer pair used by the method. The primer pair amplifies a DNA fragment containing a polymorphism site whereSSC4 g. 69991162A is larger than G; the polymorphism site where the SSC4 g. 69991162A is larger than the G is the 69991162 nucleotide from the 5' terminal on the 4 chromosome of a porcine reference genome Sscrofa11.1; the porcine reference genome Sscrofa11.1 is a porcine reference genome sequence in GenBank, wherein the updated date is February, 2017. The method can perform early screening on to-be-selected pigs, so that the problem of long time for selecting good breeding pigs in actual production is effectively solved, the breeding cost is reduced, the diameters of the porcine muscle fibers are effectively reduced or increased, the method has high accuracy and low detection cost, automatic detection can also be achieved, and the practical application value in pig breeding is very high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying the diameter of pig muscle fibers and a pair of primers used therefor. Background technique [0002] Pork quality is an important economic trait of pigs, and it is closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Therefore, the research on pork quality has become an important part in the fields of meat food processing and pig genetics and breeding. As the main factor affecting pork quality, the muscle fiber diameter has also attracted more and more attention from researchers and breeders, and has become a key component of pig breeding programs. part. [0003] However, the muscle fiber diameter can only be measured after slaughter, making breeding difficult. Molecular breeding can carry out breeding work by screening molecular markers associated with traits to avoid slaughter. Therefore, it is ver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张龙超王立贤王立刚刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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