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A SNP molecular marker affecting pig muscle fiber diameter and its application

A molecular marker, muscle fiber technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of DNA detection difficulties, achieve convenient and efficient screening, solve the inability to measure in vivo, detection efficiency boosted effect

Active Publication Date: 2022-04-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The total number of DNA in these intervals is more than 91 million. The number of DNA is too large, which makes it very difficult to detect DNA mutations in these intervals.
There are few reports of SNPs in these intervals that are significantly associated with porcine muscle fiber diameter

Method used

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  • A SNP molecular marker affecting pig muscle fiber diameter and its application
  • A SNP molecular marker affecting pig muscle fiber diameter and its application
  • A SNP molecular marker affecting pig muscle fiber diameter and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the muscle fiber diameter size of identification pig

[0030] 1. Determination of porcine SSC4g.69991162A>G polymorphism site

[0031] (1) Using two Beijing black pigs as experimental materials, the genomic DNA of the ear margin tissue was extracted respectively.

[0032] (2) Design and synthesis of primers

[0033] According to the sequence of pig reference genome 11.1, the following primers were designed and synthesized:

[0034] U (upstream primer): 5'-TCTCCACAAATTAAACTACCGTGTT-3' (SEQ ID No.1);

[0035] D (downstream primer): 5'-AGGTGAGGTTGCCCTGCTT-3' (SEQ ID No. 2).

[0036] (3) PCR amplification

[0037] The genomic DNA of the Beijing black pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain PCR amplification products, which were named product 1 and product 2, respectively.

[0038] PCR amplification system: Genomic DNA 200ng, 10×PCR amplification buffer 5μl, dNTPs final conc...

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Abstract

The invention relates to a SNP molecular marker affecting pig muscle fiber diameter and application thereof. The SNP site of the SNP molecular marker affecting pig muscle fiber diameter corresponds to the 69991162th base polymorphism site on chromosome 4 of the international pig reference genome Sscrofa11.1 version from the 5' end. The present invention also provides a primer for identifying the above-mentioned SNP molecular marker. Through the molecular marker and the primer, an efficient and accurate molecular marker-assisted breeding technique can be established. By optimizing the dominant allele of the SNP molecular marker, pig muscle fibers can be selected. The genetic progress of density traits can effectively improve the economic benefits of pig breeding.

Description

technical field [0001] The invention relates to the technical field of biological breeding, and in particular provides a SNP molecular marker affecting the diameter of pig muscle fibers and an application thereof. Background technique [0002] Pork quality is an important economic trait of pigs, and it is closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Muscle fiber diameter, which is the main factor affecting pork quality, has also attracted more and more attention from researchers and breeders, and has become a part of pig breeding programs. [0003] Molecular breeding can carry out breeding work by screening molecular markers associated with traits to avoid slaughter. In the prior art, the QTL of pork muscle fiber diameter has also been mapped and analyzed, and this trait QTL has been mapped to the 82.7-129.0Mb interval of chromosome 2, the 81.7-121.6Mb interval of chromosome 4 and the 0.6-5.7Mb interval of chromosome 18. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张龙超王立贤王立刚刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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