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Method for identifying muscle fiber density of pigs and primer pair used in method

A primer pair, muscle fiber technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficulty in breeding work, achieve automatic detection, reduce breeding costs, or reduce The effect of muscle fiber density

Active Publication Date: 2019-08-13
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, muscle fiber density can only be measured after slaughter, making breeding difficult

Method used

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  • Method for identifying muscle fiber density of pigs and primer pair used in method
  • Method for identifying muscle fiber density of pigs and primer pair used in method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 identifies the muscle fiber density size of pig

[0040] 1. Determination of polymorphism site of pig SSC14g.85531863C>T

[0041] (1) Using two Beijing black pigs as experimental materials, the genomic DNA of the ear margin tissue was extracted respectively.

[0042] (2) Design and synthesis of primers

[0043] According to the sequence of the pig reference genome Sscrofa11.1, the following primers were designed and synthesized:

[0044] U (upstream primer): 5'-CTGCACCTGACTCTTTCCC-3' (shown in sequence 1);

[0045] D (downstream primer): 5'-GGTCCACAGATTCCCCTTCC-3' (shown in SEQ ID NO: 2).

[0046] (3) PCR amplification

[0047] The genomic DNA of the Beijing black pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain two PCR amplification products, which were named product 1 and product 2, respectively.

[0048] PCR amplification system: Genomic DNA 200ng, 10×PCR amplification buffer ...

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Abstract

The invention discloses a method for identifying the muscle fiber density of pigs and a primer pair used in the method. The primer pair is used for amplifying DNA fragments containing SSC14g.85531863C>T polymorphism sites; the SSC14g.85531863C>T polymorphism sites are nucleotides from the 5' end to the 85531863 site on the 14th chromosome of a pig reference genome Sscrofa11.1; the pig reference genome Sscrofa11.1 has a pig reference genome sequence which is updated in a GenBank in February 2017. The method can be used for screening the pigs to be selected in an early period, the problem that the time for selecting high-quality breeding pigs in actual production is long is effectively solved, the breeding cost is reduced, and the muscle fiber density of the pigs is effectively reduced or increased; the method has high accuracy, a low detection cost, can realize automated detection and has a high practical application value in terms of pig breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying the density of pig muscle fibers and a pair of primers used therefor. Background technique [0002] Pork quality is an important economic trait of pigs, and it is closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Therefore, the research on pork quality has become an important part in the fields of meat food processing and pig genetics and breeding. The muscle fiber density, which is the main factor affecting pork quality, has also attracted more and more attention from researchers and breeders, and has become a key component of pig breeding programs. part. [0003] However, muscle fiber density can only be measured after slaughter, making breeding difficult. Molecular breeding can carry out breeding work by screening molecular markers associated with traits to avoid slaughter. Therefore, it is ver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 张龙超王立贤王立刚刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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