Method for detecting biodistribution of nano drug delivery system in organism
A nano-drug loading and biological technology, which is applied in the direction of drug combination, pharmaceutical formulation, analysis materials, etc., can solve the problem of lack of analysis methods such as nano-carriers
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Embodiment 1
[0042] The novel label-free laser desorption / ionization mass spectrometry imaging technique (LDI MSI) of the present invention is used to study the experimental flow chart of nanocarrier in situ drug release, refer to figure 1 .
[0043] The concrete implementation process of experimental flow process of the present invention is as follows:
[0044] (1) Synthesize single-layer molybdenum sulfide sheets by butyllithium chemical exfoliation, and modify them with PEG to increase the water solubility and biocompatibility of molybdenum sulfide nanosheets. The π–π stacking interaction between nanomaterials allows doxorubicin to be loaded on molybdenum sulfide sheets. And the mass ratio of the drug to the molybdenum sulfide was measured by means of ultraviolet spectroscopy.
[0045] (2) The drug-loaded nanomaterials were injected into the mice through the tail vein. According to the arrangement of the specific experimental group, three mice were assigned to each group as a parallel...
Embodiment 2
[0051] The LDI MSI technology of the present invention is used to image and analyze the sub-organ distribution of molybdenum sulfide nanosheets in spleen tissue. According to the specific process described in Example 1, the spleen tissue of normal mice was taken out after 24h, and the sub-organ distribution of molybdenum sulfide nanosheets in the spleen tissue could be obtained. figure 2 . Such as figure 2 b) and 2c), compare figure 2 In the optical picture of a), it can be seen that the molybdenum sulfide nanomaterials have obvious differences in the red matter and white matter of the spleen, and the distribution in the red matter is obviously more than that in the white matter, and the distribution in the boundary between the two regions is the most. figure 2 d) and 2e), show two representative mass spectra in the white matter and red matter of spleen tissue, respectively.
Embodiment 3
[0053] Distribution of molybdenum sulfide nanomaterials in various tissues of H22 subcutaneous tumor model mice.
[0054] In this example, the H22 subcutaneous tumor model needs to be constructed, and the specific steps are as follows: 1×10 7 H22 tumor cells were dispersed in 200 μL PBS solution, and then injected subcutaneously in the axilla of Kunming mice. After about 7 days of growth, the subcutaneous growth volume was about 250mm 3 of solid tumors. At this time, the nanomaterials of molybdenum sulfide were injected into the above-mentioned tumor model through the tail vein. After 24 hours, each tissue was taken out for LDI mass spectrometry imaging, refer to image 3 , it can be seen that in the subcutaneous tumor model, molybdenum sulfide nanomaterials are mainly accumulated in the lung, liver, and spleen, and less distributed in the kidney, heart, brain, and H22 tumors.
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