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A kind of composition and application thereof for preparing myocardial patch

A composition and patch technology, applied in the fields of biomedicine and medical devices, can solve the problems of lack of ability to scavenge ROS, unsatisfactory state of cell growth, difficult myocardial infarction sites, etc., and achieve good market prospects and social welfare value. , excellent performance, the effect of improving mechanical ductility

Active Publication Date: 2021-07-13
嘉兴莱普晟医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, electrospun nanofibers, such as PHBV, PANi, and PVDF, are usually used as scaffold materials for myocardial patches. Although such materials can obtain stable patches with high porosity, the biocompatibility of the above materials The sex is long, and the state of cell growth on it is not ideal
In order to overcome these problems, there is a prior art that uses a combination of gelatin-based hydrogel and polypyrrole to prepare a scaffold. Although it has good cell compatibility and electrical conductivity, its mechanical properties are poor and it is easily broken during the preparation process. , and the above materials themselves do not have the ability to scavenge ROS, which makes it difficult to better restore the activity of the myocardial infarction site in vivo

Method used

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  • A kind of composition and application thereof for preparing myocardial patch
  • A kind of composition and application thereof for preparing myocardial patch
  • A kind of composition and application thereof for preparing myocardial patch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 is used to prepare the preparation of the composition MA-Gelzan-Gly-Go-Ito-DA of myocardial patch

[0032] 1. Preparation of MA-Gelzan-Gly blend

[0033] Weigh 2 g of gelatin (Sigma), put it into 20 ml of PBS solution, heat it in a water bath at 60 ° C, stir it by magnetic force for 40 min, and dissolve it fully to obtain a 10 w / v% gelatin solution; dissolve 0.8 ml of glycerin in 20 ml of distilled water, mix Evenly, to obtain a 4v / v% glycerol solution; Weigh 1g of G3251 (a plant gel produced by Phytotechlab, CAS: 71010-52-1), put it into 10ml of PBS solution, heat it in a water bath at 60°C, and Stir for 30 minutes, fully dissolve, and obtain a 10w / v% G3251 solution; mix the above-mentioned 10w / v% gelatin solution, 4w / v% glycerin solution, and 10w / v% G3251 solution in a volume ratio of 1:1:1 Mix, heat in a water bath at 60° C., and magnetically stir for 1 h to obtain a MA-Gelzan-Gly blend.

[0034] 2. Preparation of modified MA-Gelzan-Gly hydrogel

[00...

Embodiment 2

[0043] The influence of the proportioning of embodiment 2 different gelatin, glycerin, gellan gum on the mechanical properties of MA-Gelzan-Gly-Go-Ito-DA myocardial patch material

[0044] 1. Preparation of different types of myocardial patch materials

[0045] In order to explore the influence of different ratios of gelatin, glycerin, and gellan gum on the mechanical properties of the MA-Gelzan-Gly-Go-Ito-DA myocardial patch material, 3 different gelatin, glycerin, and gellan gums were designed in the embodiment, Specifically as described in Table 1:

[0046] Table 1 Myocardial patch materials prepared in different volume ratios of gelatin, glycerin, and gellan gum

[0047] Myocardial patch material model Volume ratio MA-Gelzan-Gly-Go-Ito-DA-a 1:1:1 MA-Gelzan-Gly-Go-Ito-DA-b 1:1.2:1 MA-Gelzan-Gly-Go-Ito-DA-c 1.2:1:1.5

[0048] The preparation method of MA-Gelzan-Gly-Go-Ito-DA myocardial patch material is as described in Example 1, 10w / v% ge...

Embodiment 3

[0054] The influence of the graphene oxide of embodiment 3 different content on MA-Gelzan-Gly-Go-Ito-DA myocardial patch material mechanical property and conductivity

[0055] 1. Preparation of different types of myocardial patch materials

[0056] In order to explore the effect of graphene oxide on the mechanical properties and electrical conductivity of MA-Gelzan-Gly-Go-Ito-DA myocardial patch material, when the ratio of gelatin, glycerin and gellan gum was 1.2:1:1.5, a 4 different graphene oxide contents, specifically as described in Table 2:

[0057] Myocardial patch materials prepared by different contents of graphene oxide in table 2

[0058] Myocardial patch material model The ratio of MA-Gelzan-Gly hydrogel solution to graphene oxide solution MA-Gelzan-Gly-Go-Ito-DA-c 4:1 MA-Gelzan-Gly-Go-Ito-DA-d 4:1.5 MA-Gelzan-Gly-Go-Ito-DA-e 4:2 MA-Gelzan-Gly-Go-Ito-DA-f 4:2.5

[0059]The preparation method of MA-Gelzan-Gly-Go-Ito-DA myocar...

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Abstract

The present invention relates to a composition and application thereof for preparing a myocardial patch, the composition for preparing a myocardial patch comprises: 10-100 parts of gelatin, 4-100 parts of glycerin, and 5-50 parts of gellan gum , 3-10 parts of graphene oxide, 0.6-5 parts of dopamine, and 0.5-20 parts of itugluside. Adding gellan gum, graphene oxide, dopamine, methacrylic anhydride, and itugluside not only improves the electrical conductivity of the composition, but also improves the mechanical ductility, and at the same time, it also enables the material to have the effect of scavenging free radicals.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and medical equipment, and in particular relates to a composition for preparing a myocardial patch and an application thereof. Background technique [0002] Ischemic heart disease, as one of the main health problems in our country, has brought a heavy economic burden to our country and other countries in the world. Cardiomyocytes are terminally differentiated cells that are difficult to regenerate after necrosis. Although the current drugs, interventional and surgical treatment of ischemic heart disease can effectively improve myocardial blood supply and save dying myocardium, they have no repair effect on non-functional myocardium that has caused death. Severe cardiac arrhythmias, heart failure, and cardiac arrest can be caused by massive myocardial death. For the treatment of advanced heart disease, the more effective method is myocardial transplantation. However, the number of donors for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/08A61L27/38A61L27/50A61L27/52A61L27/54
CPCA61L27/08A61L27/20A61L27/222A61L27/3834A61L27/50A61L27/52A61L27/54A61L2300/232A61L2300/416A61L2430/20C08L5/00
Inventor 李建辉郑俊辛立明杨子江黄光涛毕会民胡刚良
Owner 嘉兴莱普晟医疗科技有限公司
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