Wound repair material for cell suspension transplantation and preparation method of wound repair material

A technology of wound repair and cell suspension, applied in medical science, prosthesis, etc., can solve the problems affecting the speed and quality of wound healing, high fluidity of transplantation, shedding of epidermal cells, etc., to reduce the formation of granulation tissue and optimize Pore ​​structure, effect of reducing wound contracture

Active Publication Date: 2019-08-20
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the one-step transplantation of simple epidermal cell suspension has high fluidity, and cannot be well confined to the wound surface and evenly distributed on the wound

Method used

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  • Wound repair material for cell suspension transplantation and preparation method of wound repair material
  • Wound repair material for cell suspension transplantation and preparation method of wound repair material

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Embodiment 1

[0034] This embodiment provides a wound repair material, which is prepared according to the following steps: dissolving type I collagen in water, and preparing 50 g of an aqueous collagen solution with a pH of 4.68 and a mass fraction of 0.35 wt%. 4.86mL, 3.6mg / mL chondroitin sulfate aqueous solution was slowly added dropwise to the above collagen aqueous solution to obtain a collagen-based composite solution with a mass-to-feed ratio of 10:1, wherein the amount of chondroitin sulfate added was 9.09wt%. After aliquoting the above collagen-based complex solution, freeze and centrifuge at 4°C and 8000 rpm for 10 min to obtain a translucent gel-like precipitate, which was diluted to 25.5 g with the supernatant. The diluted gel-like precipitate was transferred to an aluminum mold and smoothed, pre-cooled in a -60°C ultra-low temperature refrigerator, and then transferred to a -50°C lyophilizer for 18 hours of freeze-drying. After taking it out, transfer it to a vacuum drying oven ...

Embodiment 2

[0041] This embodiment provides a wound repair material, which is prepared according to the following steps: take type I collagen and dissolve it in water to prepare 50 g of an aqueous collagen solution with a pH of 3.2 and a mass fraction of 0.55 wt%. The α-elastin hydrolyzate was slowly added dropwise into the above aqueous collagen solution to obtain a collagen-based composite solution, wherein the added amount of α-elastin was 8 wt%. The above-mentioned collagen-based composite solution was transferred to an aluminum mold, scraped flat, pre-cooled in a -60°C ultra-low temperature refrigerator, and then transferred to a -50°C freeze dryer for 18 hours of freeze-drying. After taking it out, transfer it to a vacuum drying oven with a vacuum degree of -0.1MPa and a temperature of 105°C, and perform dehydration thermal crosslinking treatment for 24 hours to obtain a thickness of 2mm and an area density of 1.95mg / cm 2 , Collagen sponge with a porosity of 98%.

[0042] Using CO ...

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Abstract

The invention discloses a wound repair material for cell suspension transplantation and a preparation method of a wound repair material. The wound repair material comprises a collagen base layer prepared from 3D porous collagen sponge by tabletting treatment, the collagen sponge is prepared from a collagen-based compound solution by crosslinking treatment, the collagen-based compound solution comprises type I collagen protein and a composite, and the composite is elastin or anion mucopolysaccharide. The collagen base layer in the wound repair material for cell suspension transplantation can beused as a support stent for epidermal cell adhesion, proliferation and migration to provide a proper microenvironment for wound healing, the wound repair material can rapidly close donor sites, shallow burn wounds of large-area burn patients and shallow wounds caused by chronic skin ulcer by combining autologous epidermis cell suspension and has better application prospects in cell suspension transplantation.

Description

technical field [0001] The invention relates to the technical field of medical wound repair, in particular to a wound repair material for cell suspension transplantation and a preparation method thereof. Background technique [0002] In order to solve the shortage of autologous skin sources in patients with extensive burns, techniques such as mesh skin grafting, stamp skin grafting, small skin grafting, and micro-skin grafting have been used for many years to expand the skin coverage area, but the area of ​​skin expansion by these methods is still limited. With the development of tissue engineering technology, epidermal cell culture and transplantation technology has emerged in recent years, but the mechanical properties of the cell membrane are poor, the requirements for the operator are high, and the adhesion to the wound is poor, and the culture period is long, which greatly limits its scope of clinical application. The non-cultured autologous epidermal cell suspension i...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/24A61L27/38A61L27/50A61L27/56A61L27/58A61L27/60
CPCA61L27/20A61L27/227A61L27/24A61L27/3813A61L27/50A61L27/56A61L27/58A61L27/60C08L89/00C08L5/08
Inventor 刘曦谭荣伟陈滢滢许孟强郭远军王丹妍梁嘉美佘振定
Owner SHENZHEN QIKANG MEDICAL DEVICES
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