Tumor cell three-dimensional model and construction method and application thereof

A technology of tumor cells and construction methods, which is applied in the field of three-dimensional models of tumor cells and their construction, and can solve the problems of high cost of cell scaffolds

Pending Publication Date: 2019-08-23
GUANGDONG UNIV OF TECH
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Problems solved by technology

[0004] In view of this, the present invention provides a three-dimensional tumor cell model and its construction method and application, which are used to solve the problem of high cost of using existing materials as cell scaffolds

Method used

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  • Tumor cell three-dimensional model and construction method and application thereof

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Embodiment 1

[0054] In this embodiment, the preparation of a three-dimensional model of tumor cells includes the following steps:

[0055] 1) Preparation of sodium alginate solution and calcium chloride solution

[0056] Weigh the sodium alginate powder and add it to the reagent bottle and add deionized water, then put it into a magnetic stirrer, put it on the magnetic stirrer and stir for 12h to 24h, and the prepared mass concentrations are 3%, 3.5% and 4% respectively. sodium alginate solution.

[0057] Weigh the calcium chloride powder and add it to the reagent bottle and add deionized water to prepare a 0.1mol / L calcium chloride solution.

[0058] Put the sodium alginate solution and the calcium chloride solution together into a high-temperature and high-pressure sterilizer for sterilization at 125° C. for 15 minutes, and the preparation of the sodium alginate solution and the calcium chloride solution is completed.

[0059] 2) Planar culture of prostate cancer cells (PC3) and fibrob...

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Abstract

The invention belongs to the technical field of tumor cell three-dimensional culture, and particularly relates to a tumor cell three-dimensional model and a construction method and application thereof. According to the construction method, an alginate solution is added into a mixed cell suspension solution containing tumor cells and fibroblasts, a mixed cell-alginate solution is obtained, and thenthe mixed cell-alginate solution is dropped into a calcium salt solution to obtain mixed cell microspheres. The tumor cell three-dimensional model and the construction method and application thereofhave the advantages that alginate and calcium salt are adopted for cross-linking to prepare the microspheres as cytoskeletons, thereby reducing the cost of three-dimensional culture of the tumor cells, the alginate has good biocompatibility and non-immunogenicity, thereby being capable of providing a good three-dimensional growth environment for the cells, and three-dimensional co-culture is carried out on the tumor cells and the fibroblasts, thereby being capable of simulating the living environment of the tumor cells in the body more accurately, so that screening and/or evaluation of antitumor drugs by the constructed tumor cell three-dimensional model are/is more accurate and reliable, thereby being capable of reducing the cost of the research and development of the antitumor drugs.

Description

technical field [0001] The invention belongs to the technical field of three-dimensional culture of tumor cells, and in particular relates to a three-dimensional model of tumor cells and its construction method and application. Background technique [0002] At present, drug detection based on two-dimensional cell culture models is still the main method in tumor research. However, this method cannot accurately simulate the complex growth environment of tumor cells, so drug evaluation using a two-dimensional cell culture model is inaccurate, and a cost-effective model that can more accurately simulate the growth environment of human tumors should be constructed to replace the two-dimensional cell culture model. Cell models are critical for drug development and evaluation screening. [0003] To improve the accuracy and reliability of anticancer drug evaluation, it is necessary to change the traditional in vitro cell culture model. According to some current studies, three-dimen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071C12Q1/02
CPCC12N5/0697G01N33/5011C12N2502/30C12N2502/1323C12N2533/74C12N2513/00C12N2503/04G01N2500/10
Inventor 汤亚东苗小敏陈家盈陈巧桐李卓刚梁大锡罗竣仁张焜
Owner GUANGDONG UNIV OF TECH
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