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A kind of SNP molecular marker related to chicken leg skin hair follicle density traits and its detection method and application

A technology of skin hair follicles and molecular markers, applied in the field of poultry genetic engineering, achieves great economic value and scientific research value, saves production costs, and accelerates the effect of genetic development

Active Publication Date: 2022-05-10
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Regarding its role in the growth and development of chicken skin hair follicles, there are few relevant reports; and in the prior art, there is no relevant report on the SNP molecular markers related to chicken leg skin hair follicle density traits

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  • A kind of SNP molecular marker related to chicken leg skin hair follicle density traits and its detection method and application
  • A kind of SNP molecular marker related to chicken leg skin hair follicle density traits and its detection method and application

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Embodiment Construction

[0018] The present invention will be explained in detail below in conjunction with the embodiments.

[0019] The SNP molecular marker associated with chicken leg skin hair follicle density traits of the present invention, said molecular marker is located at the 500th position of the nucleotide sequence shown in SEQID NO: 1, and the nucleotide at this position is A or C, resulting in this single nucleotide polymorphism at a genetic locus. PCR amplification obtains the SNP molecular marker associated with chicken leg skin hair follicle density traits, said SNP molecular marker comes from Wnt3a gene, its nucleotide sequence is as shown in SEQ ID NO: 1, the A at the 500th base of this sequence / C mutation.

[0020] For the above-mentioned SNP molecular marker, the present invention also discloses its acquisition method, comprising the following steps:

[0021] (1) PCR amplifies chicken genomic DNA to be tested, obtains PCR amplification product;

[0022] (2) Sequencing the PCR ...

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Abstract

The invention provides a SNP molecular marker related to chicken leg skin hair follicle density traits and its detection method and application. The nucleotide sequence of the molecular marker is shown in SEQ ID NO: 1, and the nucleotide sequence of SEQ ID NO: 1 The 500th position is A or C. The invention provides a SNP molecular marker related to chicken leg skin hair follicle density traits, which can be used for early selection in family breeding, and can also provide molecular basis in matching. The SNP molecular marker of the present invention provides a new method for screening the hair follicle density traits of chicken legs, can save production costs and accelerate genetic development, better serve the breeding of high-quality broilers, and has great economic value and scientific research value.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic engineering, and more specifically relates to a SNP molecular marker related to chicken leg skin hair follicle density traits, detection primers, an acquisition method and application. Background technique [0002] The traditional consumption of high-quality broiler chickens in my country is mainly live chickens. Consumers are mainly related to the inherent quality of products through simple and intuitive characteristics such as feather color appearance, shank color, and crown development of chickens. Up to now, most of the new broiler breeds and matching lines that have passed the national examination and approval are the types that focus on the appearance of feathers and cater to the live chicken market, and pay little attention to the carcass appearance of the chicken skin after slaughter. However, the H7N9 epidemic has brought a devastating blow to my country's high-quality chicken ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 姬改革束婧婷章明邹剑敏单艳菊屠云洁刘一帆巨晓军张笛肖芹盛中伟
Owner JIANGSU INST OF POULTRY SCI