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A kind of method for propagating and cultivating ribbon grass

A ribbon grass multiplication and cultivation technology, which is applied in the field of plant cultivation, can solve the problems of large environmental impact and low multiplication coefficient, and achieve the effects of consistent environmental conditions, simple cultivation process and simple formula

Active Publication Date: 2021-07-20
四川立德种苗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems in the prior art that the breeding of ribbon grass is greatly affected by the environment and the multiplication coefficient is low, the present invention provides a method for breeding and cultivating ribbon grass. Through the method of the invention, the multiplication coefficient can reach 15-20

Method used

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  • A kind of method for propagating and cultivating ribbon grass
  • A kind of method for propagating and cultivating ribbon grass

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for propagating and cultivating ribbon grass, comprising the following steps:

[0026] A. Sterilization of explants: Take the stalks of Ribbon grass free of diseases and insect pests, remove the leaves, cut the stalks into 2cm segmented stems, place them on the ultra-clean workbench, and sterilize them with 75% alcohol for 5 seconds, 0.1% L Mercury sterilization for 5 minutes, rinse with sterile water for 5 times;

[0027] B. Start culture: Inoculate the sterilized explants obtained in step A in the start medium, the start medium is MS+0.2mg / L NAA+1.5mg / L 6-BA+30g / L sucrose+6g / L agar, adjust the pH to 6.0. The light time was 14h / d, the light intensity was 2500LX, and the culture temperature was 24°C. After 14 days of culture, the axillary buds began to germinate. After 40 days, the germination rate was 98%, and the axillary buds were 2-4cm high.

[0028] C. Proliferation culture: cut off the axillary buds that grow to 2-4cm in step B, and transfer them to th...

Embodiment 2

[0031] A method for propagating and cultivating ribbon grass, comprising the following steps:

[0032] A. Sterilization of explants: Take the stalks of Ribbon grass free of diseases and insect pests, remove the leaves, cut the stalks into 2cm segmented stems, place them on the ultra-clean workbench, and sterilize them with 75% alcohol for 5 seconds, 0.1% L Mercury sterilization for 5 minutes, rinse with sterile water for 5 times;

[0033] B. Start culture: inoculate the sterilized explants obtained in step A in the start medium, the start medium is MS+0.1mg / L NAA+1.3mg / L 6-BA+30g / L sucrose+6g / L agar, adjust the pH to 6.0. The light time is 14h / d, the light intensity is 2500LX, and the culture temperature is 24°C. After 17 days of culture, the axillary buds begin to germinate. After 40 days, the germination rate is 90%, and the height of the axillary buds is 1-2cm.

[0034] C. Proliferation culture: cut off the axillary buds that grow to 1-2 cm in step B, and transfer them t...

Embodiment 3

[0038] A method for propagating and cultivating ribbon grass, comprising the following steps:

[0039] A. Sterilization of explants: Take the stalks of Ribbon grass free of diseases and insect pests, remove the leaves, cut the stalks into 2cm segmented stems, place them on the ultra-clean workbench, and sterilize them with 75% alcohol for 5 seconds, 0.1% L Mercury sterilization for 5 minutes, rinse with sterile water for 5 times;

[0040]B. Start culture: inoculate the sterilized explants obtained in step A in the start medium, the start medium is MS+0.3mg / L NAA+1.8mg / L 6-BA+30g / L sucrose+6g / L agar, adjust the pH to 6.0. The light time was 14h / d, the light intensity was 2500LX, and the culture temperature was 24°C. After 14 days of culture, the axillary buds began to germinate. After 40 days, the germination rate was 88%, and the height of the axillary buds was 1-3cm.

[0041] C. Proliferation culture: cut off the axillary buds that grow to 1-3 cm in step B, and transfer th...

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Abstract

The invention belongs to the technical field of plant cultivation, and in particular relates to a method for propagating and cultivating ribbon grass. The specific steps include collecting and sterilizing the explants, initiating culture to germinate axillary buds, proliferating culture to induce clustered buds, rooting culture to obtain rooted seedlings of Ribbon grass, hardening and transplanting. Among them, the germination rate of priming culture is 98%, the multiplication coefficient of multiplication culture is 15-20, the rooting rate of rooting culture is 100%, and the survival rate of transplanting is 100%. The invention has the advantages of simple steps, convenient operation, high survival rate, and is not affected by seasonal climate changes and natural disasters, and can carry out large-scale industrialized breeding and breeding of seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for propagating and cultivating ribbon grass. Background technique [0002] Ribbon Grass (scientific name: Phalaris arundinacea L.var.picta L.), alias: Phalaris arundinacea L.var.picta L., alias: Phalaris arundinacea L.var.picta L., is a variant of Phalaris arundinacea, a perennial herb of the genus Phalaris arundinacea. Most of the stems are solitary, and some are clustered. The leaves are flat and green There are white stripes in between, which are soft and ribbon-like, hence the name. The panicles are tight and narrow, the branches are straight upward, dense spikelets, glabrous or micro-hairy, rough on the glume along the ridge, and very narrow on the upper part. wing. It is an excellent pasture for livestock when young, and has strong regeneration ability after harvesting or grazing. The stalks can be used for weaving utensils or papermaking. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张艳谢松林陈雨
Owner 四川立德种苗科技有限公司