Method for identifying activity of cotton seeds

Abstract

The invention discloses a method for identifying the activity of cotton seeds. The method comprises the following steps of: (1) soaking a piece of filter paper by water, and paving the soaked filter paper into an incubator; (2) putting cotton seeds to be tested which are soaked by water on the filter paper of the step (1) in a form of a single layer, covering another piece filter paper which is soaked by the water and without dropping on the cotton seeds, and counting the germination rate of the cotton seeds after dark culture; during culturing, spraying the water onto the filter paper covering the seeds to soak the filter paper without dropping; estimating the activity of the cotton seeds according to the germination rate. A double-layer filter paper germination method has the main innovation points that (1) the germination stage of cotton can be conveniently observed in a morphological way; (2) matrixes such as sands and soil are not needed; (3) the method is suitable for the ion culture, and can not be interfered by other matrix factors.

Description

technical field [0001] The invention relates to a method for identifying the vitality of cotton seeds. Background technique [0002] Seeds play a very important role in agricultural production, and the germination rate directly affects the production and benefits of crops. The level of seed germination rate is determined by the vigor of the embryo in the seed and the storage of sufficient endosperm. Generally speaking, the germination rate of seeds guaranteed by high vigor and sufficient endosperm nutrition is high, and the germination rate is low on the contrary. [0003] When sowing in normal soil conditions, saline-alkali land, and dry land, all seedlings need to be sown at one time. Uneven seedling emergence and difficulty in growing seedlings during cotton sowing have always been a major problem in cotton planting, which seriously restricts the promotion and development of cotton, and limits the process of cotton industrialization. Abiotic stress factors (such as dro...

AI Technical Summary

Problems solved by technology

[0005] The traditional seed germination test is the soil germination method and the sand germination method. With the development of the times, these two methods can no longer meet the needs of the development and scientific research of modern cotton production.

The most prominent disadvantage of the soil germination method is that it occupies a relatively large space, wastes land resources, reduces land productivity, and cannot quickly identify the seed vigor. In addition, the soil germination method is generally carried out outdoors, which is easily affected by the weather and the site.

The disadvantage of the sand culture method is that it occupies a large space, requires a large container and cannot be cultivated on a large scale. The sand culture method needs to wash, dry and sterilize the sand, which wastes resources, is not conducive to environmental protection, and takes up too much space. , which is not conducive to large-scale identification

And the soil germination method and the sandy soil germination method are not conducive to taking out the rhizome completely, it is easy to break, and it is easy to miss energetic but low-vigorous seeds. influences

[0006] The traditional identification of salt tolerance and drought resistance requires a lot of space, a lot of manpower and material resources, and is easily limited by environmental and climatic conditions. It is impossible to carry out large-scale accurate and fine identification, and it has been unable to meet the needs of modern cotton breeding and industrialization.

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