Pseudomonas veronii composition and application thereof in treatment of materials containing dyes

A Pseudomonas and dye technology, applied in the field of sewage biological treatment, can solve the problem of low dye degradation rate and achieve good treatment effect and good removal rate

Active Publication Date: 2019-08-27
宋金龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides the composition of Pseudomonas orthodonii and its application in the treatment of materials containing dyes, so as to solve the problem of low degradation rate of triphenylmethane dyes in water and soil in the prior art

Method used

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  • Pseudomonas veronii composition and application thereof in treatment of materials containing dyes
  • Pseudomonas veronii composition and application thereof in treatment of materials containing dyes
  • Pseudomonas veronii composition and application thereof in treatment of materials containing dyes

Examples

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Embodiment 1

[0025] This example is used to illustrate the methylotrophic bacillus and its properties of the present invention.

[0026] The preservation number is CGMCC No.17523, and it is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee. LB medium, LB medium contains tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, cultured to 10 8 The concentration of viable cells was obtained to obtain the Pseudomonas oxysperi bacteria liquid. Wherein, the nucleotide sequence of the 16S rDNA of Pseudomonas orthodonii is shown in SEQ ID NO.1.

[0027] Add 3mL of the above-mentioned Pseudomonas 150mL MSM culture medium into 150mL MSM medium, shake and culture at 30°C, 180 rpm, and take samples every 4h to measure the OD of the bacteria in the fermentation broth 650 value. The results showed that the OD of the fermentation broth at 0, 4, 8, 12, 16, 20, and 24 hours 650 They are 0.12, 0.23, 0.32, 0.34, 0.35, and 0.33, respectively. This sh...

Embodiment 2

[0035] This example is used to illustrate the microbial inoculant and its performance.

[0036](1) In 100 parts by weight of the medium, in the medium, 0.45g of corn steep liquor, 1.50g of peptone, 1:15 of bean cake powder soaking juice, 1.50g of glucose, pH 7.5, inoculated with 5 parts by weight of Bacillus licheniformis Bacterial liquid, the Bacillus licheniformis was purchased from the China Agricultural Microorganism Culture Collection Center and numbered 01050, cultivated in the same LB medium as above to 10 9 licheniformis bacterium liquid, cultivated at 37°C, sampled during the cultivation and observed by microscope direct counting method until the number of viable bacteria of Bacillus licheniformis was 2×10 8 per gram of culture medium.

[0037] (2) In the culture medium of 100 parts by weight, in this culture medium, bean cake powder 50g, sucrose 20g, ammonium sulfate 5g, trisodium citrate 2.5g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.5g, ferrous sul...

Embodiment 3

[0041] The embodiments of the present invention are used to illustrate the microbial inoculum and its performance.

[0042] According to the method of Example 2, the Pseudomonas orthodonnae, Bacillus licheniformis, and Kourtella chinensis obtained respectively in steps (1) to (3) are mixed according to the ratio, the difference is that the mixing ratio is such that In the microbial bacterial agent obtained, in terms of the number of viable cells, relative to each part of the Pseudomonas oleiferi, the content is 0.3 parts, the content of the Bacillus licheniformis is 0.1 parts, and the content of the Kwai Kueteri is 0.1 part. The content of bacteria is 0.1 part, and the microbial bacterial agent of the embodiment of the present invention is obtained.

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Abstract

The embodiment of the invention discloses a pseudomonas veronii strain. The pseudomonas veronii strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 4th, 2019, the preservation number is CGMCC No. 17523, and the preservation address is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The embodiment of the invention further provides a microbial agent. The microbial agent comprises kurthia huakui, bacillus licheniformis and pseudomonas veronii in claim 1. On the other hand, the embodiment of the invention further provides a process of treating sewage and soil which contain triphenylmethane dyes by the microbial agent. The pseudomonas veronii disclosed by the embodiment of the invention can be used for performing degradation treatment on the triphenylmethane dyes in the sewage and the soil of the triphenylmethane dyes in water bodies or soil, is good in treatment effect, and has very good removal rate especially for malachite green, crystal violet or gentian violet.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of sewage biological treatment, in particular to a composition of Pseudomonas wordneri and its application in treating materials containing malachite green dye. Background technique [0002] Malachite green is a common triphenylmethane dye, which is widely used in the textile printing and dyeing industry, and because it has a good control effect on fish saprolegniasis and other diseases, it has been used in aquaculture in my country for a long time. medicine use. Since the 1970s, a large number of reports have confirmed that malachite green has potential teratogenic, carcinogenic and mutagenic effects, and its residue in aquatic products will pose a threat to human health. In view of the harmfulness of malachite green, my country clearly lists malachite green as a banned drug. However, due to reasons such as low price and good bactericidal effect, there are still a small number of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/38C12R1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/34C12N1/20C12N1/205C12R2001/38
Inventor 宋金龙韩刚穆迎春李乐王亚妮杨臻何雅静何泽
Owner 宋金龙
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